Their morphological and physiological characteristics were observed through the strains colony morphology , size , color , growth rate , texture , and spores 用察氏平板培养基分离菌株，根据菌株的菌落形态、大小、颜色、生长速率、质地、生长培养基颜色变化以及菌丝体和抱子的形态特征进行鉴定。
Meanwhile the depiction of mycelia could contribute the identification among species . eight extracellular anzymes of mycelia from phellinus igniarius cultured by shaking were studied for the first time 同时通过对不同生长时期菌丝体形态的观察描绘，为桑黄的品种鉴定提供依据，可避免因品种混杂而影响桑黄的生物活性。
But because of having not mastered the growth conditions of mycelia and not succeeded in culturing fruit bodies , it was retarded the pace of developing and utilizing the new medical source 但由于对其菌丝体的生长条件还没有完全掌握，子实体的栽培技术还未成功，致使对桑黄各方面的研究进展很慢，阻碍了这一新兴中药资源的开发进度。
The dna fingerprinting patterns in this study are made to help identification of wild matsutake resources and their tissue isolates under various ecological environments fit for the artificial taming of matsutake 鉴于目前松茸菌丝体分离培养困难，制备松茸子实体的dna指纹图谱，为鉴定不同生境下获得的组织分离物之真伪提供了遗传依据。
The mutation may associate with the gene which resulted in degeneration of the strain . the isozymes of the cordyceps militaris and its degenerative strain on the stage of mycelia were analysed by polycrylancide gel electrophoresis 采用聚丙烯酰胺凝胶电泳技术对菌丝体阶段的蛹虫草cordycepsmilitaris ( l . ) link及其退化菌株进行了同工酶分析。
The experimental results showed that the content of se - component in the fruitbodies would be obviously promoted when na2seo3 was added to the media , but the growth and development of mycelia and fruitbodies were procrastinated and restrained to a certain extent 试验结果表明，培养基中添加亚硒酸钠时，能明显提高子实体中硒的含量，但对菌丝体和子实体的生长有一定的延迟与抑制作用。
24 samples including matsutake basidiocarps and different origin isolates were analyzed with rapd fingerprinting comparison using 17 arbitrary decamer nucleotide primers . the results showed that all slow - growing mycelia isolated from lamellae have the same dna fingerprinting 试验还对松茸菌根、带菌丝土壤进行了分离，结果很容易获得各种快生型的丝状真菌，因此认为菌根、土壤并不适合分离松口蘑菌丝体。
In the study of m . purpureus , the optimum conditions for its medium composition and culitivation were discussed . high density fermentation was carried out and the mycelium yield reached to 4 . 09g / l , which is about two times higher than the data of 2 . 18g / l reported in literature 在红曲霉的研究中，我们进行了红曲霉的培养基和培养条件的优化实验，并根据该实验结果进行了高密度发酵，最终红曲霉菌丝体的含量达到了4 . 09g l ，比我们所看到的2 . 18g l高出近一倍。
The results showed that lamellae were fit tissue for mycelia isolation , and success percentages of mycelia isolation with media pdas , pdaw , bm , pda are 74 . 4 % , 35 . 5 % , 15 . 6 % and 8 . 9 % respectively . the results also showed that stipe , mycorrhizae , and soil with the fungi are not fit for tricholoma matsutake isolation 另外，马铃薯葡萄糖麦数滤汁培养基( pdaw ) 、 bm培养基、 pda培养基也从菌褶2曾东方博士论文:腐生与共生食用菌菌丝体分离、培养及其dna鉴定研究分离到松茸菌丝体，分离成功率依次是35 . 5 % 、巧
In order to investigate the tolerance of ectomycorrhizal fungi to heavy metals in vitro , three culture methods , namely liquid culture without agitation , liquid culture with agitation and solid agar culture , were investigated to determine which method would give the best combination of fungal biomass and ec50 . the results indicated that liquid medium without agitation was the best culture method 为研究外生菌根真菌本身对重金属污染的耐性，比较了液体静置、液体摇床和琼脂固体培养这三种常用的菌丝体的纯培养方法，以真菌生物量大小和分离难易程度为主要指标，筛选出液体静置方法为最优方法。
