The gene targeting vector was planning to be constructed by using thrombopoietin gene ( tpo ) gene as purpose gene , as 1 - casein gene as targeting gene , the 5 " sequence of - casein gene as promotor 以绵羊-酪蛋白基因的5调控区为启动子，人血小板生成素基因为目的基因，奶牛s1 -酪蛋白基因为靶向基因。
The choice of the outside source purpose gene , the choice of the carrier and the preparative disposal of inoculated organization etc . are all main factors that affects the nucleic acid vaccine to express in the body 外源目的基因的选择、载体的选择、接种组织的预处理等都是影响着核酸疫苗在体内表达的主要因素。
The low expression level of purpose gene is the main problem in the current study of mammary gland bioreactor , due to the random insertion and position effects of integrated genes 在转基因乳腺生物反应器的制作中，由于目的基因在与宿主基因组整合中存在位置效应和基因串联效应，导致目的蛋白基因表达水平低、不稳定。
Currently used plant transformation systems require selectable marker genes encoding antibiotic or herbicide resistance , along with the gene of interest , to select transformed cells from a large population of mostly untransformed cells 摘要在目前的植物转化系统中，要求在关注基因或目的基因转入细胞时，同时有标记基因存在。
Objective : to construct prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen , and express the target proteins by iptg induced in escherichia coli 目的：构建截短的乙型肝炎表面抗原分子的原核和真核表达重组质粒，然后分别在大肠杆菌中诱导表达并纯化表达蛋白及在真核细胞中表达目的基因，并检测其抗原特性。
7 herbicide resistant plants were obtained . pcr , pcr - southern blotting analysis showed that the target genes were integrated into the plant genome . the segregation ratio in t1 of two examed transformants was 3 : 1 通过对标记性状? ?除草剂抗性的活体及离体检验(氯酚红法) 、目的基因pcr扩增、 pcr - southern杂交等方法证实目的基因已整合到小白菜基因组中。
A pair of primer designed according to multi - sequence comparing results of published ibv sequences in genbank was used to amplify the nucleocapsid gene of 5 strains , the amplified rt - pcr product of all 5 strains is 1 . 2kb in length 对3个克隆质粒中的目的基因片段进行序列测定，结果表明该片段含有ibvn基因全序列，其结构基因全长1227bp ，编码409个氨基酸。
In order to explain the special function of the genome , hem refers a new method which can conclude the function of the gene through analysis to the cis - regulatory elements in upstream of the core promoters 摘要为了实现基因组中特定基因功能的注释，研究者提出一种新的思路，即利用对目的基因启动子上游的顺式元件的功能的分析，进一步来推断目的基因的功能。
Results : sds - page showed that after being induced with 0 . 5 % methanol foi 48 hours , the expressed product , a 20 kda glycoprotein that was secreted into the medium , existed in supernatant in the form of soluble molecule 5甲醇诱导后，目的基因己成功表达，而且目的基因表达量随诱导时间延长而增加。经0 5甲醇诱导表达后，培养液上清中检测到表达产物。
With the development of in vitro culture and shoot regeneration from leaves , the method of agrobacterium - mediated transformation through leaf discs was widely adopted , and genes of interest have been transformed into strawberries 摘要随着植物离体再生技术的日益成熟和多种外源目的基因的分离与克隆，转基因技术以目的性强、周期短等优点成为草莓品种改良的重要途径。
