目的基因
例句与用法
- These results suggested that dgp1 could drive target gene expressed mainly in guard cells when plant is subjected to drought stress
这些结果证明，在植物遭遇干旱胁迫时， dgp1启动子可以驱动目的基因在保卫细胞中特异性表达。 - ( 2 ) the interest gene in pcar is under the control of acti which is the strong promoter in rice . the selectable marker gene is hyg gene
( 2 )表达载体pcar中目的基因由单子叶强启动子水稻act1启动子调控，选择标记基因为潮霉素抗性基因。 - Then the amplified mtb8 . 4 and ms gene were subcloned into the unique nhe i and mlu i cloning sites of pci - neo expression vector
重组质粒pm 、 pms转染ps15细胞，进行稳定表达， g418筛选抗性克隆后，用rl ’ - pcr检测目的基因在mrna水平的表达。 - Confirmation of transformant . integration of sag - ipt into chrysanthemum genome was confirmed in two thirds of the putative tansgenic plantlet be pcr amplification
通过pcr扩增表明，转基因植株中可以检测出有目的基因( ipt基因)的存在，目的基因已成功地插入菊花的基因组中。 - Inducible guard cell - specific promoters can play an important role in driving target gene expression at specific position in given conditions such as drought stress
这一目标的实现，需要诱导型的保卫细胞特异性表达或强表达启动子的创建，使目的基因在特定的条件下特定的部位表达。 - According to rt - pcr results , all the three targeted genes in chloroplast transgenics were expressed at a significantly higher level compared with those in nuclear transgenics
通过rt - pcr进一步检测表明叶绿体型转基因烟草中目的基因的表达水平明显比核转化植株中相应基因的表达水平高。 - Recombinant cloning vector of pgem - hc and pgem - ha were constructed by ligating the hc and ha genes into pgem t east vector and transformating into jm109 . 3
分别将目的基因hypoderminc和hypodermina与克隆载体( pgemteasy )连接并转化到宿主菌jm109中，构建了重组克隆载体pgem - hc和pgem - ha 。 - These include the ability to target the gene of interest to site - specific areas of the chloroplast genome , high - level expression of foreign genes and increased environment security
由于植物叶绿体表达系统能够高效表达目的基因、环境安全性高，已成为目前植物生物反应器研究的热点之一。 - 4 ) fragement recovery of pcr products pcr products were separated on a 1 . 5 % agarose gel . the expected fragment was recovered by using pcr fragment recovery kit according to the manufacturers instructions
4 、凝胶回收应用pcr目的基因片段回收试剂盒对扩增阳性pcr产物进行凝胶回收，纯化目的dna片段。