To detect the translation product , we expressed ha99 in e . coli and generated specific antibody . western analysis was performed against infected hzaml cells , bv and odv 为了检测其表达产物，进行了ha99的原核表达并制备了多克隆抗体，对病毒感染的hzam1细胞、病毒的bv和odv进行了蛋白杂交检测。
By western analysis using a chicken - derived polyclonal antibody the product of hal22 was found in infected cells as a 21kda protein , close to the theoretical size of 21 . 6kda 用原核表达的蛋白免疫鸡，得到特异性的鸡源多克隆抗体。 westernblot结果显示hsnpv感染的细胞中有ha122蛋白，分子量为21kda 。
The components of 37kd and 39kd glycoproteins from gel were immunized respectively mice and polyclonal antiserums were obtained . glycoproteins of sperm cell surface can be examined using igg rabbit anti mouse - colloidal gold as probe 用它们分别免疫小鼠得到多克隆抗体，以兔抗鼠igg胶体金15nm为探针，检查此两种蛋白在精细胞质膜上的分布。
Anti - cdc25c rabbit polyclonal antibody was purchased from santa cruz biotechnology , anti - cdc2 and anti - cyclinbl mouse monoclonal antibody were from neo - markers , anti - cdc2 ptyrls monoclonal antibody was obtained from new england biolabs 免疫印迹分析用抗体： cdc25c免多克隆抗体购于santacruz公司。 cdcz ，周期素m鼠单克隆抗体购于neomarkers公司。
Confocal microscopy observation followed immune - fluorescence staining with anti - gp130 showed that gp130 could interact with tle1 at the cytomembrane region . moreover , this interaction inhibited the concentration of tle1 into nucleus 为了进一步证实上述发现，我们表达并纯化了gst - gp130胞浆区融合蛋白和gst - tle1独特性片段融合蛋白，并制备了特异性抗tle1多克隆抗体。
In order to increase sensitivity , we take advantage of polyclonal antibody in the assay to measure the at - iii content in blood serum . the specificity of the assay can be improved by using monoclonal antibody as enzyme - linked antibody 我们所建立的双抗夹心elisa测定血清中at -含量的实验方法，采用多克隆抗体作为包被抗体，以捕获更多的at -分子，提高实验方法的灵敏度。
A plant expression vector was constructed by following method : s gene of 1bv and 35s promoter was cut from recornbinant pbi121 , and then the fragment was inserted into the multiclonal sites hind / bamh i in the plasmid pcambia1305 . 1 通过从重组质粒pbi121上切下s基因(连同35s启动子)片段，将该片段定向克隆到pcambia1305 . 1质粒的多克隆位点hind 、 bamh之间，构建了一种植物表达载体。
The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak . 8 . bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent 将克隆到的hbmp基因通过适当的酶切插入到转移载体质粒pbac - pak8的多克隆位点中，获得重组转移载体质粒pbacpak - hbmp 。
3 . preparation of polyclonal antiserum against angiotensinogen in order to study agt in protein level and also for other continuing work , the c - teminus of rat angiotensinogen was expressed in e . coll . rabbits were immunized by this expressed 6his - agtc protein and serum from different rabbits were raised 血管紧张素原多克隆抗血清的制备为研究agt蛋白表达及后续工作，在大肠秆菌中融合表达了agt蛋白的c末端，以此融合蛋白为抗原免疫家兔，制备抗血清。
Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid , and induced with methanol to secrete interesting peptide . the supernate of pichia pastoris culture was analysed by sds - page and western blotting . a reactive band , which the apparent molecular weight is 36kd , can be detected with sheep anti - hcmv polyclonal antibodies 重组质粒转化巴氏毕赤酵母， g418筛选出多拷贝插入的单克隆，甲醇诱导多拷贝插入的单克隆酵母细胞分泌目的蛋白，培养液上清经sds - page电泳分析，在蛋白质印迹中检测到培养液上清有一表观分子量为36kd ，能与羊抗hcmv多克隆抗体发生发应的条带。
