Expression of gst - snail fusion protein in prokaryotic cells and preparation of polyclonal antibody against snail 融合蛋白的原核表达及其多克隆抗体制备
To acquire the polyclonal antibody of hbaf53 , we made use of several molecular cloning methods 为了获得hbaf53的多克隆抗体,本实验采用基因工程的方法制备抗原。
Preparation of polyclonal and monoclonal antibodies against crf and change of crf in the sleep - deprived rat brain 单克隆抗体和多克隆抗体制备及其在睡眠剥夺模型大鼠脑内的变化
Preparation , purification and identification of polyclonal antibodies of vibrio alginolyticus and outer membrane proteins of vibrio alginolyticus 溶藻弧菌菌体及其外膜蛋白多克隆抗体的研究
Two specific antibodies against fl ( aa 164 - 677 ) and against f2 ( aa 1 - 159 ) were raised in chicken 基于此我们制备两个特异地作用于片段( aal64677 )和fz片段( aall59 )的鸡源多克隆抗体。
Then the fr - 008 pks produced in e . coli was used as antigen to raise polyclonal antibodies against the natural fr - 008 pks 接着将大肠杆菌表达的pks作为抗原制备与天然fr - 008pks对应的多克隆抗体。
The recombinant vector was digested with tthllll and the lacz gene from e . coli was inseted in this site , the generated plasmid is designated as pltk - uni 然后定向亚克隆swha基因于多克隆位点,获得重组转移载体pltk - ha 。
Antibodies polycolonal human , mouse , rat anti - neuron specific enolase , polycolonal mouse anti - glial fibrillary acidic protein Nspecificemlase ,博士德公司人免抗小鼠胶质纤维酸蛋白多克隆抗体( polycolonalmouseni 4ialibrill叨acidiccrotein ,博士德公司) 。
Compared with the known antibodies , the antiserum we prepared is highly specific and rules out the possibility of cross - reacting with angiotensin fragments . 4 与己有抗体相比,本实验制备的抗agt多克隆抗血清不仅具有较高的特异性,而且排除了与血管紧张素短片段的交叉反应。
In our study we have cloned the osd gene from s . typhimurium by pcr , characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd 再将hpylori尿素酶b亚单位基因与尿素酶b和热休克蛋白a融合基因分别克隆入ptrc99a一asd质粒的多克隆位点之内。