vitreoscilla造句
例句与造句
- expression of monellin gene and vitreoscilla hemoglobin gene in the methylotrophic yeast pichia pastoris according to the monellin amino acid sequence and pichia pastoris preference codon usage, the monellin gene was designed and synthesized
同时探讨vgb基因在毕赤酵母中的功能，并应用于高甜度monellin重组酵母，解决毕赤酵母高密度发酵中存在的供氧不足的问题，进一步提高高甜度monellin的产量。 - besides, vgb gene expression also increased the chitinase secretion . in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis, an inducible promoter ( levansucrase ) was cloned from b . subtilis wb600 and ligated with a promoter-less vgb gene . the resulted gene is called sacvgb and was demonstrated to express in e, coli by sds-page and carbon monoxide binding assay
由于vgb基因启动子不能在枯草杆菌中启动表达，因此，根据已发表的果聚糖蔗糖酶基因(sacb)序列设计引物，从枯草杆菌wb600总dna中扩增出该基因的启动子片段，然后将其与vgb基因编码区及终止子序列相连，成功地组建了sacvgb融合基因。 - under the induction by methanol, the expressed monellin was secreted into medium at the level of 0.25g / l . the gene ( vgb ) coding for the vitreoscilla hemoglobin ( vhb ) molecule was cloned and funtionally expressed in pichia pastoris . shake flask fermentation experiments showed that the presence of the intracellular vhb in pichia pastoris efficiently enhanced cell growth under oxygen-poor conditions
同时，采用毕赤酵母偏爱密码子，人工合成了透明颤菌血红蛋白基因(vgb)；并构建了vgb基因的酵母胞内表达载体ppic3.5kv，通过电击转化获得了能在胞内表达有生物活性vhb的重组毕赤酵母gs115/ppic3.5kv。 - for these goals, the fo llowing jobs have been done and some results have been obtained . 1 according to the vitreoscilla hemoglobin ( vhb ) amino acid sequence and plajnt preference codon usage, vgbm gene was designed and synthesized by annealing 22 synthetic fragments respectively, the modified vgbm gene of full length of 450 base pairs was synthesized . 2 the transformants were obtained after pbv221svhb was introduced into e . coli
为此本论文作了以下工作并得出了一些结论：1根据透明颤菌血红蛋白(vhb)的氨基酸序列，选用植物偏爱密码子，对透明颤菌血红蛋白基因(vgb)进行优化改造，设计并合成了22条寡核苷酸短片段，人工合成了改造的全长450bp的透明颤菌血红蛋白(vhb)基因(vgbm - a recombinant expression plasmid was first constructed by inserting the vitreoscilla hemoglobin gene ( vgb ) into the plasmid pchi, which contains the chitinase gene cloned from bacillu circulans c-2 . to study the effect of vhb on cell growth and chitinase production, the expression plasmid was transformed into escherichia coli jm109
为了进一步提高几丁酶基因在大肠杆菌的表达，将透明颤菌血红蛋白基因(vgb)插入到几丁酶基因表达质粒pchi后转入大肠杆菌jm109中，vgb基因的表达促进了转化菌的生长和提高了几丁酶基因的表达，溶氧水平越低，作用越显著。 - It's difficult to find vitreoscilla in a sentence. 用vitreoscilla造句挺难的
