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dna酶的英文

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"dna酶"怎么读用"dna酶"造句

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  • deoxyribonuclease

例句与用法

  • To establish a biochemical method to find dnaase in earthworm ; 2
    建立双胸蚓dna酶分离纯化的技术体系; 2
  • Its activity became declining when the temperature was above 70 and when the temperature is 90 , its residual activity is 38 . 4 %
    4 ,在ph7对时dna酶的活性急剧下降, ph90时剩余活性为1而出
  • The different bivalent cations with the same final concentration were put into the dnaase reacting system for 15 minute at ph5 . 4 , at 41 , then dnaase ' s activity unit was detected
    dna酶的酶促反应体系中加入终浓度相同的二价金属阳离子41下、 phs 4准确反应15分钟后测定酶的活力单位。
  • 3 . to study physical and chemical property of dnaase the purified dnaase reacted with circular pbv220 - y - inf for an hour at 37 , then the producetion was detected by 1 % agrose gel
    双胸蚓组织中dna酶的酶学性质研究将纯化的dna酶分别与环状pbv220 - ? inf37反应1小时后,用1的琼脂糖凝胶电泳对其反应产物进行观察。
  • The finding of dnaase in the earthworm the tissue extract of earthworm which had been diluted 28 times could digest 4 ul pbv220 - r - inf ( 6 . 66 u g / ul ) completely at 37 @ in a hour but it had no effect on rna
    双胸蚓组织中dna酶的发现28倍稀释双胸蚓组织粗提液37保温1小时,可将4vlpbv220 。 y一inf质粒( 6石6119 11l )完全消化但对rna无消化作用。
  • On the basis of consulting a great amount of articles about biosensor , the extraction , the purification of plant dna and the enzyme operation of dna . the preparation and application of cucumber dna voltammetric biosensor have been studied
    本文在查阅大量有关生物传感器、植物dna的提取与纯化以及dna酶切等文献的基础上,研究了黄瓜dna伏安传感器的制备及其应用。
  • The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer , high temperature , ammonium sulfate precipitation , deae - cellulose ( de52 ) chromatography and ultra - filter membrane
    双胸蚓组织中dna酶的分离纯化双胸蚓组织粗提取液经过选择性酸变性、选择性热变性、硫酸按分段盐析、 deae一纤维素( de52 )柱层析、超滤膜分级分离后得到一个电泳纯的dna酶。
  • 1 . because the taxonomic division is rather complex and has been much disputed and revised , in this part , we will review the classification and phylogeny of families , subfamilies and tribes of anseriformes based on morphology , ethology , osteology , mitochondrial and nuclear dna restriction fragment length polymorphism , single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition , classification and phylogenetic relationships of the families , subfamilies and tribes of anseriformes . the controversial questions and deficiency in the systematic studies of anseriformes were pointed out
    具体包括以下几个部分: 1 、针对雁形目鸟类异常复杂的分类状况及分类上存在的争议,根据雁形目鸟类的形态学、行为学、骨骼学、角蛋白、线粒体与核dna酶切片段长度多态、单拷贝核dna - dna杂交及线粒体基因dna序列分析等方面的研究,对雁形目鸟类分类中科、亚科和族的划分及其相互间的系统发生关系进行综述,分析系统学研究中存在的不足,提出了雁形目鸟类分类中急需解决的问题。
  • To isolate and purify dnaase in the earthworm first , the tissue extract of earthworm was prepared by dissolving the earthworm with sucrose and denaturing the protein with low ph buffer . then dnaase was purified by denaturing the protein with higher temperature . the following steps were ammonium sulfate precipitation , deae - cellulose ( de52 ) chromatography and filtration by ultra - filter membrane
    双胸蚓组织中dna酶的分离纯化采用蔗糖溶解双胸蚓,并选择性酸变性制备双胸蚓组织粗提取液,再经选择性热变性、硫酸铵分段盐析、 deae ?纤维素( de52 )柱层析及超滤膜分级分离对双胸蚓组织中dna酶进行分离纯化。
  • The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method , then the total rna digested by dnase that had not rnase was used for rt - pcr . i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition . at the end i selected the magnesium ion density as 1 . 25 mm . the production of rt - pcr was inserted directionally into pgem ? z ( ampr ) . the pgem ? z ( ampr ) was used to transform e coli jm109 . i got a positive clone through culling and identificatin . the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
    分取诱导培养液中的菌体,用异硫氰酸胍法提取总rna ,总rna再经无rna酶的dna酶处理后用于rt ? pcr 。在pcr扩增目的基因时,通过优选扩增体系,使镁离子浓度为1 . 25mm时rt ? pcr可顺利地获得目的基因,并能定向克隆到载体pgem ? 3z ( amp ~ r )中。用克隆载体转化宿主大肠杆菌jm109 ,通过筛选获取阳性克隆子,对阳性克隆子进行酶切与pcr鉴定,并对载体中插入的目的基因进行测序。
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