bacmid中文是什么意思

• 杆粒[带有杆状病毒基因组的质粒，可在细菌和昆虫细胞之间穿梭

• "bacnet" 中文翻译 :    楼宇自控
• "bacmeister" 中文翻译 :    巴克迈斯特
• "bacnotan" 中文翻译 :    巴克诺坦
• "bacmecillinam" 中文翻译 :    巴美西林; 氮卓脒青霉素羟乙碳酸乙酯
• "baco" 中文翻译 :    巴措; 巴科; 巴乔; 巴索
• "baclofen" 中文翻译 :    β-对氯苯基-γ-氨基丁酸; 巴氯芬; 贝可芬; 氯苯氨丁酸
• "baco mt" 中文翻译 :    巴科山
• "bacllus butyricus" 中文翻译 :    酪酸梭状芽胞杆菌
• "baco tone" 中文翻译 :    冻石
• "bacllus anthracis" 中文翻译 :    炭疽杆菌

• Transfection of the gp64 - mi and reintroducing hal33 gene bacmid dna into sf9 cells shown that hal33 can rescue the gp64 null bacmid in sf9 cells
  对该重组病毒bv的westernblot检测，结果表明fi和fz同时存在并以二硫键相连。
• The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme , were linked by means of t4 dna ligase and transformed into e . coli jm109 permissive cells , yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid
  将重组质粒pugedna与转移载体pfastbacldna用bamhi和ecori双酶切处理， t _ 4dna连接酶连接，用连接产物转化大肠杆菌jm109感受态细胞，得到重组转移载体质粒pfastbac - gedna 。
• The interest gene was inserted in the - tha l . tho 1 multiple cloning sites of donor plasimd pfastbachtb of baculovirus expression system . after analysis by restriction endonuclease and pcr , the recombinant donor plasmid gpl - fast was transforn1ed to the competent celi dhi0bac which contalns the bacmid and the helper plasmid , the recombinant bacmid gpl - bac was acquired which would express the vpl of gpv strain h l
  同时将该目的基因插入到杆状病毒表达系统的供体质粒pf _ ( ast ) b _ ( ac ) htb的xba 、 xho多克隆位点间，经酶切、 pcr鉴定后，将重组的供体质粒gp1 - fast转化到含有杆状病毒和辅助质粒的dh10b _ ( ac )感受态细胞中，获得了表达gpvh1株vp1的重组杆状病毒gp1 - bac 。
• I ) two mutants ( hpab - 38 and hpab - 34 ) were designed based on the three - dimension structure of hpab - constructed by protein homology modeling method , ii ) the mutant molecules were generated by pcr and inserted into pfast hta donor plasmid , the later was then transformed into escherichia coli dhlobac to generate recombinant baculoviruses dna by site - specific transposition of an expression cassette into a baculovirus shuttle vector ( bacmid ) propagated in e . coli . the recombinant bacmids were isolated and transfected into insect sf - 9 cells to reproduce baculoviruses
  4 . h队b一p及其突变体高效表达工程菌的构建:将用pcr扩增的h队b一p 、 h狱b一p35 、 h队b一p34基因，分别与pinpointxa一3质粒连接，转化jm109大肠埃希菌，获得的工程菌在表达融合蛋白时不稳定，第5代后即见不到表达条带。