噬斑的英文

• plaque

• "噬"英文翻译    bite
• "斑"英文翻译    spot; speck; speckle; stripe ...
• "噬斑法" 英文翻译 :    plaque ethods
• "混浊噬斑" 英文翻译 :    turbid plaque
• "噬斑，噬菌斑，蚀斑" 英文翻译 :    plaque
• "噬斑突变株" 英文翻译 :    plaque mutants
• "噬斑形成单位" 英文翻译 :    plaque forming units pfu
• "噬斑抑制技术" 英文翻译 :    plaque inhibition technique
• "噬斑原位杂交" 英文翻译 :    in situ plaque hybridization
• "挑取（菌落、噬斑、蚀斑等）" 英文翻译 :    picking
• "半透明型突变噬斑" 英文翻译 :    cococarde
• "噬斑还原中和技术" 英文翻译 :    plaque reduction neutralization technique
• "杂色噬斑,混杂噬菌斑" 英文翻译 :    mottled plaque
• "噬斑形成单位,蚀斑形成单位" 英文翻译 :    plaque forming unit
• "噬斑中和作用,噬菌斑中和" 英文翻译 :    plaque neutralization
• "噬白细胞作用" 英文翻译 :    leukocytophagy; leukophagocytosis
• "噬" 英文翻译 :    动词(咬) bite 反噬 make a false countercharge; hurl back an accusation; 吞噬 swallow up
• "嗜癖研究中心调查表" 英文翻译 :    addiction research center inventory
• "嗜癖研究基金会" 英文翻译 :    addiction research foundation
• "嗜癖吸毒" 英文翻译 :    addicted to drugs

• Plaque forming units , pfu
  噬斑形成单位
• In order to get the soluble recombinant eo protein and inspect the protein expression status convinently , the egfp and eo gene were ligated into baculovirus transfer vector . with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna , and plaque purification in the posttransfection procedure , the pure recombinant baculovirus were harvested , which infected the sf9 cells for amplifying to generate a p - l stock . . in the meantime , the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus . the pi - stock from a pure plaque was used to generate a high liter p - 2 stock , which was determined in liter as 1 . 14 107pfu / ml by performing a plaque assay . when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression , the strong fluorescence was obeserved on the day 3 of postinfection
  此外，为了得到可溶性重组eo蛋白并便于观察重组蛋白的表达情况，我们将egfp基因与eo基因相连插入昆虫杆状病毒转移载体中，与线性杆状病毒dna共转染sf9细胞后通过噬斑纯化得到纯的重组杆状病毒，将其感染sf9细胞制备p1种子液，同时用荧光显微镜观察绿色荧光蛋白的表达情况剔除表达效果差的重组杆状病毒。再用p1种子液感染sf9细胞制备高效价的p2种子液。通过病毒液的梯度稀释和噬斑测定，确定p2种子液的病毒滴度达1 . 14 10 ~ 7pfu ml 。