可变区基因的英文

• v-gene

• "可变"英文翻译    variable; changeable
• "区"英文翻译    H region; H
• "基因"英文翻译    gen; gene; Mendelian factor; ...
• "单链抗体可变区基因片段" 英文翻译 :    single-chain variable fragments
• "区基因" 英文翻译 :    c-gene
• "可变区" 英文翻译 :    region of variability; variable area; variable range; variable region
• "c 区基因" 英文翻译 :    c-gene
• "v-区,可变区" 英文翻译 :    v-region
• "高度可变区" 英文翻译 :    hypervariable region
• "可变区取样" 英文翻译 :    variable plot sampling
• "可变区群" 英文翻译 :    variable region group
• "可变区亚群" 英文翻译 :    variable region subgroup
• "可变区域" 英文翻译 :    variable area
• "链可变区" 英文翻译 :    gly4ser
• "轻链可变区" 英文翻译 :    variable region of light chain; variable region of the light chain
• "重链可变区" 英文翻译 :    variable region of heavy chain; variable region of the heavy chain
• "v 区基因,v 基因" 英文翻译 :    v gene
• "可变区域；变面积" 英文翻译 :    va
• "可变区域轨迹" 英文翻译 :    variable-area track
• "可变区域单元问题" 英文翻译 :    modifiable areal unit problem
• "可变基因" 英文翻译 :    variable gene
• "可变基因区段" 英文翻译 :    variable gene segment
• "可变等位基因模型" 英文翻译 :    variable allele model
• "超变区" 英文翻译 :    hypervariable region
• "多变区" 英文翻译 :    diversity region

• Cloning and expression of the variable region genes of the monoclonal antibody against human cd
  16单克隆抗体可变区基因的克隆和表达
• Cloning of variable region and signal peptide genes of anti - cd20 monoclonal antibody by rlm - race
  20单克隆抗体可变区基因及其信号肽基因
• Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library . the products were cloned into puc19 vector and their sequences were analysed . the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region , with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
  方法以从噬菌体抗体库中筛选获得的抗hbsag的fab抗体基因为模板，分别扩增出其轻、重链可变区( v _ l 、 v _ h )基因，通过重组pcr方法将轻、重链可变区基因用连接肽( gly _ 4ser ) _ 3的编码序列连接，并引入前导肽编码序列，构建具有l - v _ h - linker - v _ l结构的单链抗体基因。
• Methods : the rearranged gene fragment coding tcr y v region of the jurkat cell line was obtained by rt - pcr technique the pcr product was cloned into the eukaryocytic expressive vector pcdnas to construct pcdna3 / tcr y . after confirmed by sequncing . pcdnas / tcr y plasmids were amplified in bacteria extracted by alkaline lysismethod
  方法：本文采用rt ? ? pcr的方法扩增jurkatt淋巴瘤细胞特异性重排的tcr可变区基因片段，克隆到真表达载体pcdna _ 3中，经序列测定无误后，碱裂解法大量提取质粒，制备dna疫苗。
• In the present study , the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis . total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies . after obtained using rpas system , vh and vl genes were used to assemble scfv gene fragment with a linker primer
  应用重组噬菌体抗体库技术，从分泌小鼠抗牛精子sp18抗体的杂交瘤细胞系中分离总rna ，克隆抗体重链和轻链可变区基因，加入连接肽引物( linkerprimer )组装成单链抗体scfv ( singlechainfragmentvariable )基因并用rs引物进行扩增， sfi 、 not酶切，回收后与pcantab5e载体相连，转化e . colitg1宿主菌，构建单链抗体文库。
• However , dna damage has been ruled out as the prerequisite for alkylating agents - induced mutagenesis . for example , somatic hypermutation , which occurred around the variable region in the immunoglobin gene of b cells , is driven by antigen activation not by dna damage and is considered a kind of active mutation
  但是dna损伤并不是引起突变的必要条件，一个典型的例子就是发生在b淋巴细胞的免疫球蛋白( ig )可变区基因上的“体细胞超突变( somatichyermutation ) ” ，是由表面抗原受体而不是dna损伤驱动的主动突变。
• Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
  Mg _ 7重组噬菌体抗体库的构建及鉴定从培养的mg _ 7杂交瘤细胞中提取并分离mrna ，反转录成cdna ；利用pcr分别扩增mg _ 7单抗的重链及轻链可变区基因，并通过? dna连接子将二者连接起来形成mg _ 7单链抗体基因；将mg _ 7单链抗体基因插入pcantab5e ；将连接产物转化感受态tg1大肠杆菌，制备细菌形式的mg _ 7重组噬菌体抗体库；通过菌落计数和限制性酶切分析( ecor和hind )评估mg _ 7重组噬菌体抗体库的容量和重组率。