The he was strongly inhibited by sbti and apmsf , and very sensitive to pmsf , lbti , and tlck , while not sensitive to chymostatin , bestatin , leupeptin , tpck , pepstatin , nem and iam . all these results imply that brine shrimp he was most probably a trypsin - type serine protease . the he could be strongly inhibited by edta in a dose - dependent manner , and 50 mmol / l edta exhibited more than 56 . 5 % inhibition 对孵化酶纯化样品进行生化性质和酶性质分析发现，卤虫孵化酶的最适反应温度约为40 ，最适ph为8 . 5左右；该孵化酶对p - apmsf 、 sbti极为敏感，对pmsf 、 lbti和tlck也非常敏感，但对chymostatin 、 leupeptin 、 pepstatin 、 bestatin 、 tpck 、 nem和iam不敏感，表明该酶极可能是一种属于胰蛋白酶类型的丝氨酸蛋白酶。
There are at least three kinds of ep in petal : thiol - proteinase , metallo - proteinase , serine proteinase . they gather at the site between 43 kd and 66 . 2 kd ( about 52 kd ) on the substrate gel electrophoresis . the optimum ph of thiol - proteinase , metallo - proteinase is ph 6 , which are looked as acid ep , the serine proteinase has two forms , one is acid ep ( ph 6 ) , and the other is alkali ( ph 10 ) 花瓣中都至少有3种内肽酶：巯基蛋白酶、金属蛋白酶、丝氨酸蛋白酶，三者聚集在底物胶电泳胶条的43kd 66 . 2kd之间的位置，约为52kd ；前两种最适ph为6 ，是酸性内肽酶，后一种有酸( ph6 ) 、碱( ph10 )两种存在形式，既是酸性内肽酶又是碱性内肽酶。
Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus , and no transmembrane region was present , suggesting it might be a type of secretory protein . some generic and atipical n - glycosylation sites were present in clsp , suggesting that the protein might represent a glycoprotein . the clsp protein contained one cub ( clr / cls , an embryonic sea urchin protein uegf , and bone morphogenetic protein i ) domain from 39th to 164th ammo acids , which is known to be involved in protein - protein or protein - substrate interaction , and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids Clsp分子具有补体样丝氨酸蛋白酶的多种结构特征，包括36个氨基酸组成的疏水信号肤，一个cub ( clr / cls ， anembryonicseaurehinproteinuegf ， andbonemorphogeneticproteinl )结构域和一个胰酶样丝氨酸蛋白酶结构域( t汀psin一likeserineproteasedomain )和几个保守的糖基化位点等，没有发现有跨膜区的存在。
The content of soluble protein is also increased after deficit water stress , but the increasing of " belami " is more significant than that of " samantha " . 5 . after deficit water stress , in " samantha ' petals , the metallo - proteinase activities of the optimum ph 6 and the serine proteinase activities of the optimum ph 10 are increased markedly , the thiol - proteinase activities of the optimum ph 6 is decreased markedly 失水胁迫后显著提高了‘萨蔓莎’花瓣中最适ph6条件下金属蛋白酶、最适月季（ rosahybrde ）切花瓶插期间内肽酶与衰老的关系及失水胁迫对其影响的研究ph条件下的丝氨酸蛋白酶的活性，显著降低了最适ph6条件下琉基蛋白酶的活性。
The changes of these endopeptidase are different during the former vasing of " samatha " and " belami " . the main eps in petals of " samatha " are the acid thiol - proteinase , metallo - proteinase and the alkali serine proteinase and the main eps in petals of " belami " are the acid thiol - proteinase and serine proteinase . during the later vasing the acid metallo - proteinase and the alkali serine proteinase become main eps in petals of " samatha " and " belami " 这3种内肽酶在两种切花瓶插初期(盛开以前)的表现不同： ‘萨蔓莎’花瓣中以酸性金属蛋白酶、巯基蛋白酶和碱性丝氨酸蛋白酶起主要作用， ‘贝拉米’花瓣以酸性巯基蛋白酶、丝氨酸蛋白酶起主要作用，后期‘萨蔓莎’和‘贝拉米’花瓣均以酸性金属蛋白酶、碱性丝氨酸蛋白酶起主要作用。
The full orp encodes a 487 - amino acid protein with a calculated molecule weight of 53 . 484 kda and an isoelectric point of 6 . 75 . at the primary sequence level , it shared high homology with clr , so was named clr - like serine protease , clsp . the clsp sequence has been submitted to genbank / embl ( genbank accession number af178985 ) 该蛋白在核酸和氨基酸水平上与人补体组分cl :表现高度同源，故将其命名为补体cl :样丝氨酸蛋白酶(旦lr一like旦erineprotease ， clsp ) ，该基因已经在ge心ank登录(登录号为af17s985 ) 。
The enzyme activity could be completely inhibited by pmsf and dfp , and partially inhibited by pepstatin a , edta and egta . leupeptin , benzamidine and hydrochloride aprotin had no influence on the dhap activity . ca2 * , mg2 + and na * could slightly increase , but cu2 + and zn2 + slightly inhibited protease activity 此结果表明， dhap为一种丝氨酸蛋白酶，其酶活性的发挥需要某些金属离子的参与，如na + ， c扩+ ， m扩+等金属离子能提高dhap的活性，但c了+与z矛十能抑制dhap的部分活性。
Almost one - third of all proteases can be classified as serine proteases , including complement subcomponent clr / cls , mannose - associated serine proteases ( masps ) , ovochymase , spermadhesin , type ii transmembrane serine proteases ( ttsps ) etc . these proteins are involved in diverse biological processes , including developmental processes such as complement activation , ovulation , fertilization , tissue remodeling , cellular migration , cancer invasion and metastasis , intestinal digestion , embryogenesis , or organogenesis 丝氨酸蛋白酶( serineprotease )是机体最重要的酶分子之一，约占机体蛋白酶的三分之一，我们较熟知的丝氨酸蛋白酶就包括补体组分c1r c1s 、甘露糖结合丝氨酸蛋白酶、 ovochymase 、 spermadhesin和型跨膜丝氨酸蛋白酶等，它们参与了补体活化、排卵、授精、组织重建、细胞迁移、肿瘤浸润和转移、消化、胚胎发育、器官形成等多项生理功能。
Although sds - page displayed that there were several protein bands in the sample after 16h fermentation , enzymatic active stain demonstrated that there was only one band during the whole fermentation period , indicating that there is only one kind alkaline protease in the fermentation liquid 对发酵液进行sds - page和蛋白酶的活性染色分析表明，发酵液中的蛋白质种类不多，且只含有一种碱性蛋白酶。该发酵液所含蛋白酶活性能够被丝氨酸蛋白酶抑制剂pmsf ， dfp完全抑制。
After careful studying their relative importance to immune response and the possibility of the match , seventeen sequences of interest were selected for further experiment , including estss analogous to 11 . 5kd antibacterial peptide , lysozyme , serine protease and its inhibitor , lectin , antifreeze protein , et al . primers designed according to the sequences were used to amplify the corresponding estss from both blood and cephalothorax cdna library 在仔细分析了它们在免疫系统中的重要性和在对虾中出现的可能性之后，从中选出了17条可能编码抗菌肽，溶菌酶，凝集素、丝氨酸蛋白酶及其抑制剂，抗冻蛋白等蛋白质的序列，以此为依据设计引物，在中国对虾的血液和头胸部cdna文库中扩增相应的序列。
