We used the icr and kunming mice and got the embryos of 3 . 5dpc by means of superovulation , then cultured the embryos on the feeder layer derived from icr or kunming mice , dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time . in this period , we analized the effects of feeder layer , trypsin and embryos isolated from uteri of different varietal mice on the es cell lines 以icr小鼠和昆明小鼠为实验对象，采用超数排卵的方法获得小鼠胚胎，培养在用丝裂霉素c处理过的icr小鼠和昆明小鼠mef细胞饲养层上，选取适当时间离散增殖的icm和类es细胞集落，分析了在小鼠es细胞建系过程中，饲养层细胞、消化液及不同品系来源的胚胎的影响。
The primary primordial germ cells is obtain from the human embryos after 4 - 8 weeks of pregnancy . after mechanical desection and enzymic digestion , the cells were cultured on mouse embryonic flbroblast or human embryonic flbroblast feeder layer inactivated by mitomycin . the medium contains several cytokines : lif ( leukemia inhibitory factor ) , bfgf and foskolin 从4 ? 8周的流产胎儿的原始生殖嵴部位分离到原始生殖细胞，经过机械和化学方法的解离后培养于事先用丝裂霉素处理过的小鼠胚胎成纤维细胞或人胚胎成纤维细胞饲养层上，培养基中添加了lif ， bfgef ， foskolin等细胞因子，此后大约每7天左右传代一次。
Normal bn rats were vaccinated intraperitoneally with lctcv , ltcv and rpmi1640 culture buffer ( blank control ) respectively . one - way mixed lymphocyte reaction ( mlr ) , flow cytometric analysis of the apoptosis of peripheral t cell and the rate of cd4 / cd8 in peripheral blood were performed before and after vaccination . results mlr showed that the immune response capability of bn rat spleen cells in lctcv group were suppressed significantly after vaccination ( p < 0 . 01 ) 于疫苗接种前和每次接种后第5天以被免疫bn大鼠的脾细胞作为反应细胞，以lou c大鼠的脾细胞作为刺激细胞(经丝裂霉素处理) ，进行单向混合淋巴细胞反应( mlr ) ，同时对外周血t细胞凋亡和cd _ 4 ~ + 、 cd _ 8 ~ + t细胞亚群进行流式细胞分析。
