行标记

• Sh - sy5y cells were differentiated into neuron - like cells by ra treatment in low concentration , total rna was extracted and labeled to hybridize with the microarray , the changes on gene expression were detected to find differentially expressed genes
  首先应用低浓度的ra处理sh - sy5y细胞，促使细胞分化成神经元表型，然后提取总rna并进行标记，与制备的基因表达谱芯片进行杂交，检测基因表达变化，查找差异表达基因。