蛛丝

• Its repetitive motif typically is ( ggp ) nggx and the homology with argiope trifasciata fiagelliform silk protein mrna ( atff ) is 69 . 3 % , which is the maximum comparing with all silk genes known in ncbi genebank
  其典型重复结构是( ggp ) _ nggx 。与现有已知的蛛丝蛋白基因中三带金蛛( argiopetrifasciata )鞭毛样丝基因( atff )有最高的同源性69 . 3 。
• Construction of araneus ventricousus ampullate gland cdna library and the finds of two new spider silk genes are the bases of supplying the background of araneus ventricousus silk genes and further researching in the spider silk proteins
  大腹园蛛主壶腹腺cdna文库的构建和两个蛛丝蛋白新基因的克隆，为提供大腹园蛛蛛丝蛋白基因背景和进一步研究蛛丝蛋白奠定了基础。
• Then , we find out the write clones through the chemis hib . during the working , three probes which we used are from the published sequences of spidroin . after this , a genie library of subclone of dragline was constructed
  然后我们从构建蛛丝蛋白的亚克隆文库着手，采用鸟枪法测序的策略，取从蜘蛛cosmid文库中经过证实的阳性克隆scos - ds1 ，大，小为40kb左右，用cai ， eco47 ，和hinf三种酶分别对其进行酶解分析。
• The beta - sheet , alpha - helical and its second structures make it have strong strength , high tenacity and elastic extensibility . spidroin is superior to other protein fibers . these properties make it useful in medicine , especially , in tissue engineering
  由丙氨酸组成的-折叠和富含甘氨酸的-螺旋，及其紧密堆砌的二级结构使之成为一种半结晶状态的分子弹簧结构，决定了蛛丝具有强度高，韧性大等一些重要特点。
• The cdna library of major ampullate glands of araneus ventricousus was prepared successfully for the first time by using puc18 vector in the world . two genes encoding spider silk protein and one gene encoding no - silk protein were cloned by means of randomly picking colony ( bird gun )
  本研究在国内外首次构建了大腹园蛛( araneusventricosus )主壶腹腺( majorampullategland ) cdna文库，并以鸟枪法从中筛选出两个蛛丝蛋白和一个非丝蛋白cdna新基因。
• In the pansi hole , many girls are the enchanting charming evil spirit , they like pursuing stimulation with the challenge , confessing sincerely with them which makes them to be different from the common ladies , the disciple boasts in the spider ' s work , who can make any knives expiration , in addition , when they display absorbs the soul , has how many people also to be able the heart like serine water , does not get up the mighty waves
  盘丝洞中多是妖娆娇媚的魔女，她们喜欢追求刺激和挑战，坦白和率真也令她们不同于寻常女子，盘丝弟子擅长蛛丝结阵，能够令任何暗器失效，此外当她们施展勾魂摄魄的时候，有几人还能心如止水，不起波澜？
• Two spider silk protein genes ( avfl and avf2 ) screened from the library are characterized in a lot of repetitive motifs , a high guanosines or cytidines content , a strong preference for adenosine or thymidine in the third position of a codon and rich residues of glycines or alanines in the proteins translated
  试验结果，该文库容量为4 . 9 10 ~ 6 。从文库中筛选到avf1和avf2蛛丝蛋白新基因，具有典型重复序列多， g c含量高，密码子第三个碱基偏爱使用a t及编码蛋白中含有大量gly和ala残基等特点。