蚓

• Under the same condition , the tissue extract of earthworm and the tissue protein extract of earthworm could decompose the pbv220 - r - inf completely but the tissue extract of earthworm without protein could n ' t . 2
  双胸蚓组织粗提取液及双胸蚓组织的蛋白提取液在37oc保温1小时可以完全消化pbv220 p一inf质粒而双胸蚓组织去蛋白提取液对pbv220 y一wf质粒无消化作用。
• The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer , high temperature , ammonium sulfate precipitation , deae - cellulose ( de52 ) chromatography and ultra - filter membrane
  双胸蚓组织中dna酶的分离纯化双胸蚓组织粗提取液经过选择性酸变性、选择性热变性、硫酸按分段盐析、 deae一纤维素（ de52 ）柱层析、超滤膜分级分离后得到一个电泳纯的dna酶。
• Robustness of our results was confirmed by high bootstrap support of all nodes in the trees . this result contradicts the batrachia hypothesis ( a salamander + frog grouping ) , and is consistent with bolt ' s hypothesis ( 1991 ) basing on the morphological data . the result was also supported by previous molecular studies based on mitochondrial and nuclear rrna data
  这个结果与蛙类假说是相矛盾的，与bolt ( 1991 )中国泽蛙线粒体基因组结构及种群系统地理学研究在形态学基础上提出的绒蝶类和蚓螺类为姐妹群关系的假说相一致，并得到建立在线粒体和核trna基因数据基础上的许多分子研究的支持。
• To isolate and purify dnaase in the earthworm first , the tissue extract of earthworm was prepared by dissolving the earthworm with sucrose and denaturing the protein with low ph buffer . then dnaase was purified by denaturing the protein with higher temperature . the following steps were ammonium sulfate precipitation , deae - cellulose ( de52 ) chromatography and filtration by ultra - filter membrane
  双胸蚓组织中dna酶的分离纯化采用蔗糖溶解双胸蚓，并选择性酸变性制备双胸蚓组织粗提取液，再经选择性热变性、硫酸铵分段盐析、 deae ?纤维素( de52 )柱层析及超滤膜分级分离对双胸蚓组织中dna酶进行分离纯化。
• The complete nucleotide sequence of the mitochondrial genome of f . limnocharis was detailedly compared with those of 5 other amphibians . the nucleotide sequences of 22 trna encoded by 6 amphibians mitochondrial genomes were combined and aligned to the homologous sequences of the 11 veterbrate taxa . using teleosts as outgroup , the phylogenetic analyses results show that mp , nj and ml trees all strongly support the monophyly of living amphibians with respect to other living tetrapods and favor a sister group relationship for caecilians and salamanders
  我们在测定了泽蛙线粒体全基因组序列的基础上，与已知其它的5种两栖类进行详细的比较分析，同时选择了11种高等脊椎动物的线粒体全基因序列，以硬骨鱼类做外群，用22个trna基因合并数据进行系统发生重建分析，结果表明mp 、 nj和ml树都强力地支持现生两栖类动物为单系群并且蝾螈类和蚓螈类为姐妹群关系(自引导值分别为92 、 99 、 100 ) 。
• To find dnaase in the earthworm the tissue extract of earthworm with different concentration reacted with rna , circular dna , linear dna for an hour at 37 , then the producetion was detected by 1 % agrose gel . the tissue extract of earthworm , the tissue protein extract of earthworm and the tissue extract of earthworm without protein reacted with pbv220 - r - inf for an hour at 37 , then the producetion was detected by 1 % agrose gel . 2
  双胸蚓组织中dna酶的发现用不同浓度的双胸蚓组织提取液与rna 、环状dna及线状dna在37反应1小时后用1的琼脂糖凝胶电泳对其反应产物进行观察；双胸蚓组织粗提取液、双胸蚓组织蛋白粗提取液及双胸蚓组织去蛋白提取液分别与pbv220 - ? inf质粒37反应1小时后用1的琼脂糖凝胶电泳对其反应产物进行观察。