As a member of this superfamily , human acidic fibroblast growth factor ( afgf or fgf1 ) , expressed by a variety of cells from all three germ layers , shows great potential for clinical applications for therapy of a variety of diseases , such as parkinson ' s disease , spinal cord contusion injury , neural regeneration in reimplantation of broken - off finger , brain ischemia , renal ischemia , myocardial infarction , occlusive vascularitis , retinal ischemia , gastric ulcer and nonhealing wound and so on 人类酸性成纤维细胞生长因子( afgf ， fgf - 1 )是成纤维细胞生长因子超家族成员。来自于三个胚层的多种细胞都可以表达afgf 。 afgf在治疗帕金森综合症、急性脊柱扭曲性损伤、断指中神经功能重建、脑缺血、肾缺血、心肌梗塞、闭塞性脉管炎、视网膜缺血、胃溃疡及难愈合性伤口等多种临床应用方面具有巨大潜力。
In the early neurula , cortactin staining signals were detected in the developing primodium of forebrain as early as 10 . 5 hpf ( hours post fertilization ) , at the same time the tailbud areas were highly stained too . later , the staining was concentrating in the developing neural rod while paraxial mesoderm derivatives were not stained 在早期神经胚，神经系统中的前脑原基最先出现染色，并保持头尾两端处染色最强的图式，随后，染色集中分布在发育中的神经龙骨中，但近轴中胚层细胞没有染色。
Marrow stromal cells are adult stem cells from bone marrow that have the potency to differentiate into multiple nonhematopoietic lineages such as bone , cartilage , and lipocyte et al . it also can differentiate into myocytes , hepatocytes , glial cells , and neurons . marrow stromal cell can be easily obtained and have a set of well - developed separation and culture techniques 背景与目的骨髓间充质干细胞( mesenchymalstemcells ， mscs )是具有多向分化潜能的成体干细胞，除了能向多种中胚层组织细胞分化，如骨软骨和脂肪细胞外，还可以诱导分化成非中胚层的细胞组织如：神经细胞，肺泡和小肠细胞。
Human bone morphogenetic protein 3 is a member of tgf - b superfamily . lt can induce the differentiation of cartilage and bone tissue in mesenchymal cell . and is important to bone self - repairment and bone development during embryo morphogenesis . in addition , some other biological activities of hbmp - 3 have also been found . such as inducing development of embryo and stimulating differentiation of neural and blood cells . therefore , there is a great prospect in the use of hbmp - 3 . there is trace content of hbmp - 3 in human body . it has been expressed in the expression system of eukaryotes and prokaryotes respectively , but its application is restricted because of defects in the process and modification after translation in prokaryotic cells and higher costs and lower yields existed in eukaryotic expression system 人骨形成蛋白3 ( hbmp - 3 )属于tgf -超家族的一员，可以诱导间充质细胞分化为软骨和骨，在胚胎时期骨骼发育和骨再生修复中起着重要的作用，而且对胚胎发育过程中中胚层的诱导和分化、造血组织的发育以及神经系统的发育和修复等都起着重要作用，因而hbmp - 3有广阔的市场前景。它在人体内含量极微，尽管研究人员已经在原核细胞和真核细胞表达系统中分别进行了表达，但是由于原核表达系统缺乏翻译后的加工修饰，真核表达系统存在成本高、产量低等特点，限制了其在临床上的应用。
The ratio of ( s + g2 ) / ( g2 + m + s ) and pi value of mc - 3t3 - el cells increased in the condition of 1um / l e2 . the effects of e2 on igfbp - 6 and igf - 2 gene expressions were suppressed by tcdd and ra . the binding of ere at higfbp - 6 5 " - utr ( dna sequence : aactctgacc , + 94 to + 103 ) was enhanced by tcdd 第三部分igfbp一6基因5 ’一utr中ere的鉴定及功能分析目的:骨骼组织起源于胚胎期中胚层，维持正常的胚胎期骨骼发育需要多种生长因子、细胞因子和激素的参与，其中雌激素可通过多种途径作用子中胚层内间
Ranging from 100 to 200 microns across , the width of several human hairs , these microscopic fossils are surprisingly complex and constitute almost a textbook example of a bilaterian , including the three major tissue layers ( the endoderm , mesoderm and ectoderm familiar from high school biology texts ) , the presence of a gut with a mouth and anus , and paired coeloms ( body cavities ) surrounding the gut 这些微化石的大小为100 ~ 200微米，相当于几根头发的宽度， ?们看起来出奇复杂，而且简直就像两侧对称动物的教科书般，展示了各种特徵：包括三个主要的组织层（内胚层、中胚层及外胚层，都是中学生物课本中熟悉的名词） ，前后连接口部与肛门的肠腔，肠腔外还围绕著成对的体腔。
