9 colonies from 7 inbred rat strains were studied by dna fingerprint . the result indicated that the dna fingerprints had the great difference between the different strains , the average fragments were 16 . 360 + 2 . 178 , the band - sharing rate was 0 . 061 + 0 . 008 . , the . similarity coefficient was 0 . 062 + 0 . 008 and the rate having the same dna fingerprint b 6 .实验利用聚合酶链反应( . pcr )扩增技术对国内北京和哈尔滨等4家单位6个品系( shr 、 shrsp 、 lew 、 rcs 、从叹y和f344 )的8个近交系大鼠群体进行了dna多态性的分析研究。
Objective : to study the relationship between the hbv dn ' a c gene mutations and the cellular immunity of patients with chronic hepatitis b . . methods : hbv dna c gene mutations were confirmed by polymerase chain reaction ( pcr ) and sequenced the products directly ; the cytokines ( ifn - yx tnf - a and il - 2 ) levels in serum were measured by enzyme linked immunosorbent assay ( elisa ) ; the distributions of t - lymphocyte subpopulations in peripheral blood were detected by flow cytometry ( fcm ) 目的：探讨慢性乙型肝炎患者乙肝病毒( hbv ) c基因区变异与机体细胞免疫水平的关系。方法：通过流式细胞分析技术( flowcytometry ， fcm )检测外周血t淋巴细胞亚群，利用酶联免疫吸附试验( elisa )检测血清细胞因子( ifn - 、 tnf -和il - 2 )水平，采用聚合酶链反应( pcr )扩增hbvdnac基因区片段，并对pcr产物直接测序。
