Design : viable mtb were detected in mesenteric lymph nodes , spleen and lungs at day 15 , 30and 60 after intragastric administration of bcg to mice : the proliferation of t - lymphocyte with the stimulus of the purified protein derivative ( ppd ) was measured at day 60 and 90 after intragastric administration of bcg to mice ; the expression of interleukin - 2 ( il - 2 ) of t - lymphocyte in spleen was detected at day 60 and 90 after intragastric administration of bcg 方法：在小鼠胃内接种bcg的第15 ， 30 ， 60天，检测结核杆菌在小鼠体内脏器的定植；接种bcg的第60 ， 90天，检测小鼠脾淋巴细胞受到ppd刺激后转化试验小鼠脾淋巴细胞受到ppd刺激后il - 2表达量。
Objectives to improve the effect of a single mtb8 . 4 dna vaccine , we constructed a chimeric mtb8 . 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8 . 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3 . we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8 . 4 / hil12 was presented as endogenous ag 目的：以il - 12作为分子佐剂，与结核杆菌新抗原mtb8 . 4基因连接形成嵌合分子，将其克隆到真核表达质粒中，构建成嵌合dna疫苗，研究其在小鼠体内诱导细胞免疫应答的效果及对c57bl 6n小鼠的免疫保护作用，为寻求安全、有效、廉价的结核病新疫苗打下基础。
