结合位点

• Part iv computer - aided molecule modelling of tnfa mimotopes and tnfa - binding peptides : to investigate the interaction between tnfa mimotopes and tnfa - binding peptides , the computational docking program autodock ( with confirming calculations using discover ) was used to predict the binding modes of llt - 18 with tnfa firstly , then lcs - 7 was docked to llt - 18 by manual . the interaction between llt - 18 and tnfa or lcs - 7 showed electrostatic interaction and h - bond dominant
  进行对接，对两者间结合位点进行分析；在此基础之上，以insight11软件构建lcs刁分于三维结构， autodock程序对接llt和lcs 7两个短肽分子，找寻两者结合的关键性残基，结果表明llt与tnfa及lcs 7分子间相互作用以静电相互作用为主， lcs刀和tnfa活性部位的arg在分于间相互作用中起重要作用。
• In order to get some functional clues from their structures , the upstream regulation region of ndrgl gene and second structure of ndrg2 protein are performed bioinformatics analysis ; we found that there are several binding sequences of some diffirent transcription factors , their functions include regulating tissue - specific gene expression , regulating expression of genes related to growth and early development of cells , besides this , regulating expression of genes under some stimulated conditions , and so on . predict in protein fold classification shows that ndrg2 belongs to alpha / beta hydrolase fold family , and there are high similarity between ndrg2 and epoxide hydrolase from bacteria , this suggests that ndrg2 protein may has enzymatic functions associated with resisting the oxidative stress , maintaining the balance of cell redox potential , involving in the metabolism process of xenobiotics or intracellular toxic molecules
  研究发现呷基因的调控区存在多种转录因子结合位点，功能主要涉及组织特异性表达调控，细胞生长发育相关基因的表达调控，刺激反应基因的表达调控等； ndrgz蛋白在结构上属于a小水解酶类折叠，折叠分类预测表明ndrg2与其中的的细菌环氧化物水解酶的二级结构极为相似，提示ndrgz蛋白具有一定酶活性，可能参与细胞抗氧化应激反应，维持细， an ） armtbffiofbfochmilsyn ） mdafblechmrbfobo4第四军医大学硕士学位论文胞内氧还电势平衡，参与内外源有毒物质的代谢等。
• The result indicates that the mg2 + ion around the binding site is very important to stabilize the binding of the ligand to the receptor . the binding free energy estimated by the empirical function favors the system with one mg2 + ion , in which the free energy is about 5 kcal / mol less than the system with mg2 + ion
  含有mg2 +离子的情况下比不含mg2 +离子的体系之间的自北京工业大学工学硕士论文一由能之差为一5kcal m ，结果说明结合位点处的mg ‘ ”离子对稳定配体与受体的结合起了重要作用。
• By observing the effect of acidity on the uv difference spectra of ehpg or hbed , the spectral characteristics of apoovotransferrin binding to metals and anions at ph 7 . 4 show that the reaction of apoovotransferrin with metals is related to deprotonation of the phenolic group of tyrosine of the metal binding sites , while the binding of apoovotransferrin to anions is associated with the protonation of the phenolic group of tyrosine of the anion binding sites
  摘要通过观察酸度对模拟转铁蛋白小分子ehpg和hbed紫外差光谱的影响，结合脱铁伴清蛋白与金属离子、阴离子结合的光谱特性，推断脱铁伴清蛋白与金属离子的结合导致金属离子结合位点处酪氨酸酚羟基的去质子化，而与阴离子的结合导致阴离子结合位点处酪氨酸酚羟基的质子化。
• Kyot2 lacked two lim domains from the c terminus and had a frameshift in the last exon , creating the rbp - j - binding region in the c terminus . kyotl had a negligible level of interaction with rbp - j . the binding site of kyot2 on rbp - j overlaps those of ramic and kyot2 repressed the rbp - j mediated transcriptional activation by ramic by competing with them for binding to rbp - j
  在肌肉母细胞系czc12内过表达kyoth时可阻断转录激活物notch胞内区或ebnaz对rbp依赖性启动子的转录激活作用，这种阻断作用呈剂量依赖性，提示kyoth可与notch胞内区或ebnaz竞争rbp j上的结合位点，从而抑制二者的转录激活活性
• Using transfac 4 . 0 and tssg tssh nsite of softbeny , we can see that a tata box lie in - 188bp - 198bp from transcriptional initiation site , there are several api , c / ebp binding motifs near it or in other 5 " flanking region , a c - fos response element is in - 1133bp - 1143bp from transcriptional initiation site
  0软件和softbeny网站上tssg ， tssh ， nsi ’ fe软件共同分析，在a ? ibgp转录起始点上游j到一198区域存在tata盒，在其附近及其5 ’上游远端调节区存在多个api ， c ebp结合位点，并且在转录起始点上游人到一处存在一个c fos结合基序。
• Subsequently , the binding of ( 35 ) ~ s - cam and a . thaliana protoplasts were processed , including association , dissociation , competitive and saturation analysis . the results show the binding of ( 35 ) ~ s - cam and protoplasts is rapid , reversible , saturable and sensitive to proteinase ; otherwise the specificity of binding was assessed with different competitors . k _ ( d ) of 9 . 2 nm and about 25 , 000 binding sites per protoplast were gained through saturation analysis
  随后，对~ ( 35 ) s - cam和拟南芥原生质体的结合进行了受体学分析，包括结合、解离、竞争性和饱和分析，结果显示~ ( 35 ) s - cam和拟南芥原生质体的结合具有结合快速性、可逆性、竞争特异性和浓度饱和性，从饱和分析可以得出k _ d约为9 . 2nm ，每个原生质体表面大约含有25 ， 000个结合位点，并且其结合对蛋白酶敏感。
• This article mainly discusses the challenges , research approaches and recent developed tools in the field of protein function prediction and the ways by which these issues change the process of drug discovery , including homology - based annotation transfer , sequence motifs and patterns , information on 3d structure , sub - cellular localization , posttranslational modifications , binding sites and functional residues , protein - protein interactions
  摘要简述了在蛋白质功能预测领域中的研究方法和最新研发工具所面临的挑战，并讨论蛋白质功能预测是如何改变药物开发进展的，具体包括：基于序列同源性分析的注释转移、序列基元和模式增加了注释转移的说服力、 3d结构信息可以精炼注释转移、亚细胞定位、翻译后修饰、结合位点和功能残基、蛋白质之间的相互作用。