Stewarl - akers , et al . effect of ganulocyte - macrrophage colonyatimulating factor on lymphokine - activated killer cell induction . blood , 1993 ; 10 : 2671 张林生,等.脐血造血干/祖细胞集落产率的研究.中华血液学杂志, 1996 ; 5 : 233
In situ transplantation of autologous bone marrow stem cells mobilized by granulocyte colony - stimulating factor for treatment of ischemic cerebral infarction in rats 粒细胞集落刺激因子动员自体骨髓干细胞原位移植治疗大鼠缺血性脑梗死
Human granulocyte - macrophage colony stimulating factor ( hgm - csf ) is a glycoprotein that stimulates the production of granulocftes , macrophages , and white blood cell 粒细胞巨嗜细胞集落刺激因子( gm - csf )是一种造血生长因子，它参与造血调节过程。
Granulocyte - macrophage colony - stimulating factor ( gm - csf ) exists widely in the hematopoietic system , immune system and nervous system 粒细胞巨噬细胞集落刺激因子( gm - csf )广泛存在于造血系统、免疫系统和神经系统，能调节多种细胞的分化增殖。
11 pgcs were isolated from 55d goat and cloned to seconed passage , nor pgcs from 70th day goat embryonic genital . the presence of lif had no positive influence on the isolation of es - like cell 55d的胎儿获得11个pgcs集落，可传代2次，从70d胚胎的生殖腺没有分离到pgcs细胞集落。
The researchers also spliced a gene into the virus that makes it produce granulocyte - macrophage colony - stimulating factor , which induces the body ' s immune system to recognize and attack tumors infected by the virus 研究人员还向病毒中插入了一段基因使得受侵细胞分泌粒细胞集落刺激因子，这促进了机体的免疫系统识别和攻击受到病毒感染的肿瘤细胞。
The accine was constructed of lethally irradiated pancreatic cancer cells , engineered to include a granulocyte macrophage colony stimulating factor ( gm - csf ) gene that recruits immune - system cells to seek and destroy microscopic cancers 疫苗是由致命的被照射过的胰腺癌肿瘤细胞株构成，含有粒细胞巨噬细胞集落刺激因子( gm - csf )基因，从而激活免疫系统细胞发现并破坏微小癌细胞灶。
There was about 7 days interval between every two passages . eg cell clones can be found in each primary culture from those embryos of appropriate age . in culture of one embryonic cell line , eg cell clones maintained after 9 passages 在实验中，几个适龄的胚胎的原代培养物中都出现了鸟巢状的eg细胞集落，维持时间最长的一个胚胎细胞株在传代培养了9代之后仍然可以观察到eg细胞集落。
We used the icr and kunming mice and got the embryos of 3 . 5dpc by means of superovulation , then cultured the embryos on the feeder layer derived from icr or kunming mice , dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time . in this period , we analized the effects of feeder layer , trypsin and embryos isolated from uteri of different varietal mice on the es cell lines 以icr小鼠和昆明小鼠为实验对象，采用超数排卵的方法获得小鼠胚胎，培养在用丝裂霉素c处理过的icr小鼠和昆明小鼠mef细胞饲养层上，选取适当时间离散增殖的icm和类es细胞集落，分析了在小鼠es细胞建系过程中，饲养层细胞、消化液及不同品系来源的胚胎的影响。
The blastocyst was obtain from the mouse after 3 . 5 days of pregnancy , and was cultivated on the mouse embryonic flbroblast feeder layer . the blastocyst usually attach to the feeder layer after 48 hours , then the inner cell mass began to grow and form a big cell mass of embryonic stem cells . these cells can form cell clones with the conformation of embryonic stem cell 囊胚一般在48小时后贴壁并脱去透明带，由囊胚中的内细胞团增殖形成一个胚胎干细胞球，待其生长至一定程度后对其进行酶解传代，在传代后的细胞中重新生成es集落形态的细胞集落，在集落生长至一定程度后再进行传代，此后大约每5 ? 7天传代一次。
