Abstract : adopting the serum - free and animal - source - free medium domestication express cell efficiently , setting up to express system efficiently , suspending culture cell , can raise the cell density in the scale turn the production , strengthen the cell vitality , control cell to propagate level , extension cell culture period , increase the target protein of yield , raise product quality , simplification of produces technics , reduce production cost , then raising the efficiency that the scale turns culture 提要：采用无血清无动物组分培养基驯化高效表达细胞，构建高效表达系统，悬浮培养细胞，可以在规模化生产中，提高细胞密度，增强细胞活力，控制细胞增殖水平，延长细胞培养周期，增加目标蛋白的产量，提高产品质量，简化生产工艺，降低生产成本，进而提高规模化培养的效能。
