All these results demonstrated that dhap is a serine protease . the first 20 amino acid residues of the purified dhap were determined and the sequence is aqtvpygipqikapavhaqg 将纯化的dhap进行转膜，测定了n一末端的加个氨基酸残基的序列，其结果是: aqtvpygipqikap /汉haqg 。
The purity was detected by sds - page , and only igg ' s heavy chain and light chain protein tope were showed up . the activity of igg was tested by technique of immune credeschs gold 用sds - page进行igg的纯度检验，电泳结果出现两条蛋白带，是igg的重链和轻链，说明纯化的程度较高。
This may be a good method for obtaining soluble and abundant plant actin isoforms and other proteins which could not fold exactly and exist in inclusion body by prokaryotic expression 这一方法可能是解决植物肌动蛋白及其他在包涵体中表达蛋白难以正确折叠和大量纯化的有效途径之一。
Cloning , sequencing and identifying two novel cdna fragments related to chordoma are described in the first part and expression of fhit gene in chordoma is in the second part Dd一pcr产物应用凝胶系统分离、扫描，切下差异条带，重新进行pcr扩增纯化，扩增体系、条件同dd一pcr 。将纯化的。
We extractct sam from the cell by the perchloric acid , then neutralize the extraction with khco3 . using the weak - acid caion exchange column chromatography we separate sam from the mixure 本文根据sam带电性，确定了先高氯酸抽提、 khco _ 3中和，再用弱酸性阳离子交换树脂纯化的工艺路线。
The cbd tag of the fusion protein was cut by site - specific protease enterokinase at starting met of the target protein lt 27 . it was released from the cbd fusion tag efficiently 利用融合蛋白中目的蛋白lt 27与cbdtag接头处的肠激酶特异性识别位点，用肠激酶处理粗纯化的融合蛋白，可将卜
3 a new purification method of cnts was present . the cnts films were prepared with the cnts purified by the former method , and the structures and the properties of the films were measured 提出一种新的碳纳米管（ cnts ）的纯化方法以及把用此方法纯化的cnts制备成膜的过程及性质的初步探索。
But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation 将表达产物破包涵体后经glutathinonesepharose4b柱亲和层析分离， sds page显示纯化的蛋白质为一分子量60kd的单一蛋白带。
Cf series ceramic dynamic filter is an filtration equipment based on the sieving effect of more roles ceramic membrane , which achieve the aim of concentrating and purification by dynamic Cf陶瓷动态过滤机是基于多孔陶瓷膜介质的筛分效应而进行物质分离的设备，采用动态过滤方式使流体达到分离浓缩和纯化的目的。
Comparison of the entire derived peptide sequence with other serine protease revealed significant homology , especially with a reported gene from another b . pumilus strain tyo - 67 ( 99 % homology ) 将克隆到的碱性蛋白酶基因(命名为ap )编码的成熟蛋白n一端序列与纯化的脱毛蛋白酶n一端序列进行比较，二者完全相同。
