These observations show that reprogramming is easier in interspecific embryos reconstructed with es cells than that reconstructed with somatic cells , and that es cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells . oocytes were reconstructed with outbreeding kunming albino mouse es cells and enucleated rabbit oocytes , and the effects of the passages of es cells and 6 - dmap on the development of interspecific reconstructed oocytes were analyzed . the interspecific reconstructed es - rabbit oocytes were activated either by combined two set electric pulses and 6 - dmap or by two set electric pulses 以上结果显示， 6 dmap能增加胚胎干细胞异种重构卵的卵裂率，对重构卵囊胚的发育率影响不大；高培养代数和低培养代数的胚胎干细胞用于异种核移植不影响异种重构卵的卵裂率和囊胚发育率；用胚胎干细胞作为供核细胞；比体细胞作为供核细胞所构建的异种重构胚更容易进行重编程，并且胚胎于细胞指导异种克隆胚胎正常发育的能力强于体细胞。
The constitution methods of converged surface based on the shape of around surfaces are proposed . meanwhile , the key technologies of 3d reconstruction for automobile panels are especially researched , which includes : after analyzing four data sources , which are engineering drawings , product , electric information and design thought , of reconstruction and their characteristics , the methods and flows of 3d reconstruction based on the different data sources are proposed ; studied the technology of surface segmentation and splicing and blending of complicate surfaces and the difficult cases ; the design criteria and specifications of 3d model and the basic framework of designing 同时，在上述基础理论的支持下，重点研究了汽车覆盖件三维重构的关键技术，主要包括：在分析工程图、实物或模具、电子信息和设计思维四种重构数据源及其特点的基础上，提出了基于不同数据源的三维重构的方法与流程；研究了曲面分割与拼接技术、复杂曲面的倒圆技术及难点解决方案；研究并建立了汽车覆盖件三维数学模型的设计规范与技术要求以及基于正向设计和三维重构设计思路的汽车覆盖件的开发流程基本框架；提出并研究了基于二维工程图与模具型面点云的曲面重构流程和数据处理方法。
There was no significant difference between the fusion rates of reconstructed embryos . the cleavage rate of embryos reconstructed with c57bl / 6j oocytes was significant high than that of kunming albino , but the former only developed to 4 - cell stage ; the later could develop to blastocyts . it is implied that the oocytes of inbred mouse cannot support the long - term development of kunming es cell 两种重构胚的融合率之间没有显著差异( p 0 . 05 ) ， c57bl 6j重构胚的卵裂率( 60 . 5 )明显高于昆明白小鼠( 33 . 7 ) ，但是前者只发育到4细胞期，而后者可以发育到桑椹期和囊胚期( 2 . 3 ) 。
Measurement of the fragment lengths was accomplished with the use of scion image analyzed software , which allows a curved fragment to be measured in a stepwise manner at nanometer level resolution , and the change laws of three forms of dna , supercoil , open circular and linear form as the dose increased were obtained 借助于afm ，对粒子和两种重离子诱发的dna损伤进行了纳米水平的结构分析，并结合scionimage图像分析软件的分段测量方法，对辐照诱发的dna碎片长度分布进行了纳米级分辨的测量。
