The results indicate that there exists a positive cooperative effect between oecs and c17 . 2 in proliferation ( p < 0 . 05 ) ; oecs ca n ' t influence the differentiation of c17 . 2 ; oecs showed more permissiveness comparing to 3t3 2增殖、分化及生长方式的影响。结果显示两种细胞共培养中在增殖上有正协同作用（ p 0刀5 ） ； oecs对口2的分化没有影响：与3t3相比， oecs对
Preparation requires isolating the patient ' s white blood cells and then separating out a mix of dendritic cells and precursor cells still undergoing maturation ( both types may produce an immune reaction ) 其准备功夫包括先分离病人的白血球，然后从中分离出树突细胞以及未成熟前驱细胞的混合物（这两种细胞都可能产生免疫反应） ，再与融合蛋白一起培养40个小时，之后才注射回病人身上；这个步骤一共要重复三次。
Collect three groups culture supernatant for elisa experiment , rt - pcr and elisa results indicated that both transcription and translation of two cytokine genes can be enhanced obviously . rt - pcr results showed that the transcription level of il - 1 and tnf - a genes of oligochitosan contrast group were as 2 Rt pcr结果表明两种细胞因子基因转录水平明显提高，加糖对照组il一互p和tnf a基因转录水平分别为空白对照组的2
Methods : vascular endothelial cells , smooth muscle cells and fibroblast are respectively isolated from human umbilical veins by enzyme digestion and tissue plant methods , subcultured , purified and identified , etc . phase - contrast and electron microscopy was used to analyze the cells morphological characteristics 方法：分别采用酶消化法和组织块法培养血管内皮细胞、血管平滑肌细胞及成纤维细胞，并进行三种细胞的传代、纯化、鉴定以及形态学观察。
Primary cultured nscs ( labbled by hoechst33342 ) and c17 . 2 ( a kind of immortal nsc cell line expressing b - galactosidase ) were injected into the lateral ventricles of neonatal mouse , then the cells " survial , integration and migration were compared 1w and 6w after the transplantation . the results are as follows : after lw , the living cells of primary nscs were much more than c17 . 2 ( p < 0 . 01 ) , besides , the former mainly resided in the ventricle whereas the latter integrated to the parenchyma 利用此条件，在体比较了原代nscs及永尘化神经干细胞系c17 . 2相同条件下移植后的异同，即将两种细胞注射于新生小鼠侧脑室，分别于1w ， 6w后观察比较它们的存活、整合及迁移情况，结果如下： 1w时，原代nscs存活的细胞数远大于c17 . 2 ( p 0 . 01 ) ，且主要分布于脑室系统内，而c17
Here we managed to make cultured mice peritoneal macrophages be directly influenced by oligochitosan , and be stimulated by ifn - r before oligochitosan added , then measured the changes of gene transcription and translation level of both il - 1 and imf - a , respectively by methods of relatively quantitive rt - pcr and elisa . first , rt - pcr results showed that 18 hours was the most effective time and 40ug / ml was the most effective concentration of oligochitosan , then by the same method , confirm that 4hours is the most effective time and loou / ml is the most effective concentration of ifn - r stimulating . because ifn - r can enhance il - 1 and tnf - a gene expression of macrophages alone , so add ifn - r to microphages alone for 22 hours , then examined by rt - pcr , the results showed that il - 1 and tnf - a gene expression have no remarkable difference compared with the blank contrast group 此外，由于ifn y单独作用也可促进两种细胞因子基因表达，故在巨噬细胞中加入ifn y单独作用22h ，再经阿一pcr检测，发现加ifn y的实验组细胞的几一lp和tnf a基因转录水平与空白对照组相比较无显著性差异，可见，壳寡糖和ifn v对巨噬细胞il lp和tnf一口基因转录水平的影响在作用时间上无一致性，在壳寡糖作用最适时间时，仅受ifn y刺激的巨噬细胞il lp和tnf q基因转录己下降至刺激前水平，因此可以认为， ifn y的加入仅起到对巨噬细胞预刺激使之处于敏感状态的作用，有利于增强壳寡糖对巨噬细胞的作用。
Methods : in the first part , we survived and cultured m4g3 hybridoma in vitro . after two weeks of injecting hybridoma into mouse abdominal cavity , we collected abundant ascites . at the same time , 4 strains of breast cancer cells had been growing in vitro , digested and collected for immunocytochemistry and western blotting with m4g3 ascites 方法：第一部分：复苏m4g3杂交瘤细胞，体外培养，小鼠腹腔注射，进行单克隆抗体的大量制备；复苏t47d等四种乳腺癌细胞，体外培养，爬片，应用m4g3进行免疫细胞化学实验；四种细胞收集，应用m4g3进行western - blot染色。
Phopholipase c - 1 ( plc - 1 ) is widely known to play an important role in regulating cell proliferation and differentiation , development of the organisms , cell transformation and oxidative stress . till now , the mechanism how phopholipase c - 1 acts can not be thoroughly illustrated , nor has the interaction between plc - 1 pathway and other signal pathways been systematically reported . this research chose 2 - de + ms as the basic method from all kinds of proteomics strategies and compared the total protein expression map of mef genetically deficient in plc - 1 ( plc - 1 - / - ) to that of wild type mef ( plc - l + / + ) aimed to find some protein spots differentially expressed , thus we can discuss the impact of knockout of plc - 1 on signal transduction initiated by growth factors such as egf comprehensively . in this way , we can study the biological function of plc - 1 and mechanism of plc - 1 pathway indirectly , which will contribute a lot to further analysis 鉴于plc - 1发挥上述作用的机制尚未完全阐明， plc - 1通路与其他信号通路间的交联和代偿尚无系统报道，又因为以往的研究方法不够全面，本研究以野生型小鼠胚胎成纤维细胞( plc - 1 ~ ( + / + ) )和缺失磷脂酶c - 1的小鼠胚胎成纤维细胞( plc - 1 ~ ( - / - ) )为研究模型，在众多蛋白组学策略中选择了双向电泳+质谱( 2 - de + ms )作为研究手段，通过对比表皮生长因子( egf )刺激24小时后上述两种细胞的总蛋白质表达差异，全面地探讨敲除plc - 1对生长因子诱导的信号传递的影响，从而间接研究plc - 1生物学作用、信号传递机制及其代偿情况，为后续的深入研究打下基础。
Using those techniques , it is possible to reconstruct vascular model in vitro whose structure and function are same as autogenous vessels . in this experiment two degradable materials were precoated with cross - linked type i collagen , moulded into tubular porous scaffold , and then seeded with ecs . using rotary cell culture technique , vascular model was constructed in vitro 本实验采用胶原与几种可降解材料相复合，构成管形支架，在体外环境下，探索构建分别含内皮细胞、平滑肌细胞及成纤维细胞的三层结构的组织工程化血管，三种细胞相互作用、相互支持，形成一个在形态和功能与正常血管近似的组织工程化血管。
In order to probe the reason of the obvious improvement in the biocompatibility of implanted pp , the surface chemical structure was characterized . results showed that cooh + ion implantation caused the formation of some new o - containing groups , which was responsible for the enhancement of the biocompatibility of pp 离子注入后聚丙烯表面的细胞吸附性研究表明，通过对pp表面进行cooh ~ +离子注入处理，三种细胞的生长形态要比未处理的pp样品优良，这显然与表面化学结构的变化和亲水性的改善密切相关。
