The specific subjects as bellow : firstly the two aqueous two - phase system of peg / ( nh4 ) 2so4 and peg / k2hpo4 were compared , then the better system was used for the aqueous two - phase extraction 首先针对聚乙二醇硫酸铵和聚乙二醇磷酸氢二钾这两个双水相体系进行比较，选取较好聚乙二醇硫酸铵的双水相体系用于双水相萃取分离。
Through the theoretical prediction and experiment tests , electrodialysis method could be used directly to separate glutamic acid and glutamine , and a little ammonium sulfate in the mixture benefited the separation process 通过理论预测和实验验证，可以用电渗析法直接分离谷氨酰胺和谷氨酸，且原料中少量的硫酸铵有利于分离过程的进行。
Electromagnetic wave shielding fabric was prepared by in - situ chemical polymerization of aniline in the presence of cotton substrate , using hydrochloric acid as the doping agent and ammonium persulfate as the oxidant 摘要以盐酸为掺杂剂，过硫酸铵为氧化剂，在棉织物表面采用现场吸附聚合法生成一层导电聚苯胺，由此设计出一种具有良好电磁屏蔽效能的织物。
With 2 , 2 " - azobis - 2 ( amidineopropane ) dihydrochloride ( v5o ) and ammonium persulphate ( aps ) being used as initiator , the cationic and anionic nanoparticles polystyrene ( ps ) latex were synthesized via emulsion polymerization respectively 通过使用过硫酸铵和偶氮二异丁脒盐酸盐作引发剂，利用乳液聚合法制备了粒径约为100nm ，表面分别为负电性和正电性的ps (聚苯乙烯)乳胶粒。
3 . the extracellular phb depolymerase was purified from 09 by using hydrophobic column chromatography and gel filtration technique in sephadex g - 100 . the specific activity of the purified enzyme was increased by 37 . 9 folds over crude extract , and the recovery yield was 8 . 9 % 以粗酶液为起始，经硫酸铵分级沉淀、 sephadexg - 100凝胶过滤后，分离纯化了该酶，纯化倍数约为37 . 9 ，酶活力回收率8 . 9 。
Medium experiments were arranged under uniform design , and then an optimum medium was got accordingly . the culture liquid was centrifugalized at 3 , 500r / min for 30min , then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others 通过硫酸铵分级沉淀、 deaesephadexa - 50阴离子交换凝胶层析和sephadexg - 75凝胶柱层析对发酵液进行分离和纯化，并得到电泳纯的酶。
