直接注射

• The recombination gene micyoinjected is 5nl dna ( about 106 - 107 ) into everyone oocytes or cytoplasm of grass carp . there were 32197 oocytes of 12 series via micyoinjection and achived 12945 fries in 2000 year , by hatching and raising . genomic dna was extracted from grass carp who were transformated in 1120 grass carp presented postive reaction by blood
  实验通过将hu - - ifn基因重组分子采用显微注射法将这种重组基因转化草鱼1 - 2细胞期的受精卵，即以narashige显微注射仪，将5nldna (约10 ~ 6 10 ~ 7分子)直接注射到选择好的受精卵核的动物极上。