Guan xiaohong ( 1991 ) established a cell line of the monoclonal anti - idiotypic antibody ( anti - id ) np30 of schistosoma - 6 - japonicum , whose isotype was identified to be igm and which was testified to be internal image of gaa 分别以日本血吸虫单克隆抗独特型抗体wbo的杂交瘤细胞株总基因组dna和其提取rna进行rticr合成的cdna第一链为模板，扩增v 。 、 v 。
Amplification , cloning and sequence analysis of the light chain and the heavy variable region genes of monoclonal anti - idiotypic antibody np30 of schistosoma japonicum to amplify and sequence the gene of the heavy chain and the heavy chain of anti - idiotypic monoclonal antibody np30 of schistosoma japonicum 2 、日本血吸虫单克隆抗独特型抗体np30的单链抗体kcfv ）对balb c小鼠诱导保护性作用研究为了研究日本血吸虫抗独特型抗体np30单链抗体作为日本血吸虫病
Thus , immunologists have sought smaller molecules with the antigen - recognition capability of antibodies . the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable . it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues 二、日本血吸虫单克隆杭独特型抗体np30的单链狐（ scf ）的构建、表达及对balbic小鼠诱导保护性作用研究l 、日本血吸虫单克隆杭独特型抗体np0的单链抗体归cfv ）的构建、表达通过pcr方法体外扩增并经测序验证的重链、轻链可变区（ vh 、 vl ）基因先后重组入原核表达质粒ptha90相应的位点上，中间通过一连接肽（ gly在er ） 。
The expressive gene fragment was 333bp long . analyses of the tcr y fragment shpwed that it contained three epitopes . it was showed that specific anti - idiotypic antibody could be found since four weeks after the first immunization and came to the climate on the sixth week . the antibody titers of the same time were higher in group pcdna3 / tcr y - il - 2 than in group pcdna3 / tcr y ( po . 01 ) . the highest antibody titer was 1 : 640 in the group pcdna3 / tcr y - il - 2 , whereas the highest was 1 : 160 in the group pcdna3 / tcr y . there were mrna expression in skeletal muscle cell could be found in group pcdnas / tcr y and group pcdna3 / tcr y - il - 2 on fifth day after inoculation 用tcryvi独特型dna重组载体作为疫苗免疫小鼠，结果表明pcdna3汀cry组及pcdna3厅cry ? il ? 2组小鼠1血肩中全部产生了特异性抗独特ffo抗体，抗体滴度在第4周开始增高，第6周时达到高峰。在同一取血时间， w儿1 ry一几一二组小鼠抗体滴度均高于pcdna3 tcry组（ p 0 1 ） 。 pcd a3 tcry组小鼠抗体滴度最高达1 ： 16小pc 。
Guan et al ( 1991 ) established a strain of monoclonal anti - idiotypic antibody np30 ofschistosomajaponicum , which is an internal image anti - idiotypic antibody of gut associated antigen ( gaa ) , its antibody isotype is igm . np30 has a partial cross reaction with soluble egg antigen ( sea ) and membrane associated antigen ( maa ) . np30 molecule has been researched on many aspects 管晓虹等( 1991 )建立了一株日本血吸虫单克隆抗独特型抗体np30 ，已证实为肠相关抗原( gaa )的内影像抗独特型抗体，其抗体同型为igm ，与可溶性虫卵抗原( sea )及膜相关抗原( maa )有部分交叉反应。
Guan xiaohong , et al . ( 1991 ) established a strain of monoclonal anti - idiotypic antibody np30 of schistosoma japonicum , whice is an internal image antigen of gut associated antigen ( gaa ) , its antibody isotype is igm . np30 has a partial cross - reaction with soluble egg antigen ( sba ) and membrane associated antigen ( maa ) 管晓虹等建立的日本血吸虫单克隆抗独特型抗体( anti - id ， ab _ 2 ) np30是肠相关抗原( gaa )的内影像anti - id ，与可溶性虫卵抗原( sea )有交叉反应，可替代虫源性抗原用于血吸虫病免疫诊断和抗病疫苗的研究。
