6 - dmap + ccb + ethanol can effectively activate the reconstructed rat - mouse embryos . 5 ) 225 reconstructed embryos from 2 - cell to morula were transferred into 46 recipients 23 l6 dmap乙醇ccb能有效地激活种间核移植重构胚。
No pregnant rats had been observed for 20d . this event indicated mouse enucleated maturation oocytes ca n ' t support the donors of rat fibroblast cells to develop a viable offspring 将255枚2细胞以上的核移植重构肚，移植给46只sd假孕鼠，均未妊娠。
In this review , we summarize the distribution in oocyte and functions of the cytoskeleton in oocyte maturation , fertilization and development of normal and nucleus transferred embryos 本文综述了细胞骨架在卵母细胞成熟、受精、早期胚胎发育和核移植发育过程中的分布和作用。
There were no significant difference in fusion rate , cleavage rate and blastocyte development rate with different enucleation time of oocytes during ivm16 - 24h ( p > 0 . 05 ) 在体外成熟16 24h期间，水牛卵母细胞去核时间的不同对核移植后的融合率，分裂率和囊胚发育率没有显著影响。
The purposes of this experiment were to establish cuture systems of fibroblasts and methods of cell cycle synchronization so as to provide better donor cells for somatic nuclear transfer 本实验旨在建立成纤维细胞的培养系统和细胞周期同步化方法从而为体细胞核移植提供良好的供体细胞。
The results show that the development rates of morula treated with serum starvation for 3d , 4d , 5d are higher apparently than nocodazole group and control group ( p < 0 . 05 ) 直接注射法构建的核移植重组胚胎的桑堪胚发育率（ 25 95 ）高于电融合法（ 16 07 ） ，统计学分析差异显著（ p 0
Within 2h isolation from the follicle 100 % of oocytes underwent germinal vesicle breakdown and both the nucleolus and the nuclear envelope disappeared . after culturing for 6 h , prometaphase occurred in 90 % oocytes 本研究旨在探索一种崭新的、化学试剂诱导去核卵母细胞为核受体的、无透明带的、手工体细胞核移植方法。
4 . rat - bovine interspecies somatic nuclear transfer 1 ) bovine cocs had been cultured in the maturation medium containing 8 - 10 % ocs in vitro for 22 - 24h , 60 . 86 % - 61 . 41 % of maturation oocytes can be obtained （四）大鼠牛种间体细胞核移植研究的主要结果牛卵母细胞在含8 10发情牛血清（ ocs ）的成熟培养基培养22 24h ，能使60
2 ) we got 55 . 08 % of fusion rate when reconstructed embryos were fused with 1500 - 1800v / cm voltage and 50ms impulse . in addition , a same result can be obtained with low voltage + length impulse or strong voltage + short impulse 41的卵母细胞达到成熟：在1500 1800v cm ， 30 40sin条件下，大鼠牛核移植重构胚能达到50厂0 55
This means changing the genome of either a gamete ( sperm or egg ) or a zygote ( a single cell formed either by fertilisation or by somatic cell nuclear transfer , a . k . a . cloning ) so that people are born with a designed genetic alteration 这意味着改变配子（精子或卵子）或合子（由受精或体细胞核移植形成的一个细胞，核移植也称克隆） ，以至于人是由一种设计了的遗传变化而出生。
