In order to breed maize dwarf mosaic disease ? esistance maize species , the pap gene was introduced into maize inbred strains and hybrid strains mediated by pollen and particle bombardment , 10 transgenic maize plants were achieved and the pap gene was indeed integrated into the maize genome by analysis of molecular biotechnological methods . the pap gene in one of the transgenic maize plants had been transcription and translated , the ability of the virus resistance is undergoing 为了解决玉米矮花叶病害的防治问题,采用花粉介导法及基因枪法将pap基因分别导入玉米的杂交种及自交系,获得了10株转基因玉米, pcr及southern - blot检测结果表明pap基因已经整合到了玉米基因组中,并且其中有一株pap基因已经得到了表达,病毒抗性鉴定表明转基因玉米能明显推迟玉米矮花叶病害症状的表现。