H chain cdr3 region is thought to be the antigenic epitope of antibody of monoclonal antibody np30 . therefore , anti - id antibody vaccine and antigenic epitope vaccine are important strategy in developing schistosomajaponicum vaccine Fv基因，且其表达产物保留了抗体的亲和性和特异性，有可能替代杂交瘤细胞分泌的单克隆抗体np30作为免疫检测试剂。
Guan xiaohong ( 1991 ) established a cell line of the monoclonal anti - idiotypic antibody ( anti - id ) np30 of schistosoma - 6 - japonicum , whose isotype was identified to be igm and which was testified to be internal image of gaa 分别以日本血吸虫单克隆抗独特型抗体wbo的杂交瘤细胞株总基因组dna和其提取rna进行rticr合成的cdna第一链为模板，扩增v 。 、 v 。
Then protein crystal of eif - 5a was observed using atomic force microscopy . further , the polyclonal and monoclonal antibodies were gained from immunized rabbits and rats . hypusine - contained eif - 5a plays a role in cell growth and differentiation 用得到的重组蛋白分别免疫家兔和小鼠，获得兔的抗血清和三株持续分泌单抗的杂交瘤细胞株，为进一步分析eif - 5a的功能提供了检测手段。
Therefore the hybrid - hybridoma can secrete not only bispecific antibody but also anti - human rbc type a monospecific antibody and probably anti - p24 monospecific antibody . the mixed antibodies purified by ammonium sulfate is further purified by p24 affinity chromatography . the unsp 这样，分离出双特异性抗体，并证实杂交杂交瘤除分泌抗p24和人红细胞的双特异性抗体外，还分泌抗p24单特异性抗体和抗人a型红细胞单特异性抗体。
2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6 . their antibodies " isotypes are tested by goad anti - mouse isotype regent 取驯化好并处于对数生长期的2 - e _ 4 - a和s _ 2 - b细胞，再常规融合和筛选，三次克隆化后得稳定分泌双特异性抗体的杂交-杂交瘤细胞株6株。
Methods : in the first part , we survived and cultured m4g3 hybridoma in vitro . after two weeks of injecting hybridoma into mouse abdominal cavity , we collected abundant ascites . at the same time , 4 strains of breast cancer cells had been growing in vitro , digested and collected for immunocytochemistry and western blotting with m4g3 ascites 方法：第一部分：复苏m4g3杂交瘤细胞，体外培养，小鼠腹腔注射，进行单克隆抗体的大量制备；复苏t47d等四种乳腺癌细胞，体外培养，爬片，应用m4g3进行免疫细胞化学实验；四种细胞收集，应用m4g3进行western - blot染色。
It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions , clearing aged cells and metabolic products , regulating immune responses and protecting against infection . in some pathological states such as psoriasis and contact dermatitis , a certain serum level of the antibody could inhibit the progression of the diseases , and is beneficial to the recovery from the diseases . after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies , meanwhile , experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) " , a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability , immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis , and recombinant antibody is under investigating 抗角蛋白自身抗体( akautoab )是naa的重要组成部分，以往实验通过杂交瘤技术、免疫亲和层析技术和噬菌体抗体库技术分别获得单克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ，并对akautoab免疫学特性及在体生理和病理意义进行了广泛的研究，直接或间接地发现akautoab是机体正常免疫调节网络的组成部分，在维护某些生理状态的稳定、清理衰老细胞及代谢产物、调节免疫和抗感染等方面起到重要作用；在某些病理情况下(如银屑病、接触性皮炎等) ，体内akautoab的组分和滴度会发生变化，而正常水平的akautoab则有利于限制病情的发展，促进损伤的修复。
Serious cross reation existed between v . albo - atrum and mv2 , mv3 , mv4 . the other pathogen isolates v31 and v32 also had cross reactions , but the reaction was not serious . because limited number of pathogen isolates were selected , it could not prove that the selected immunogen was widely presentative , more pathogens isolates should be tested to verify the acquired hybridomas cells 5株单抗杂交瘤细胞中没有一株具有种或属的特异性，其中mv2在棉花黄萎病菌若干菌系间的检测表明其能够区分不同的致病类型； mv1和mv4组合检测的结果基本上能将棉花大丽轮枝菌鉴定到种；黑白轮枝菌与mv2 ， mv3 ， mv4的交叉反应比较强烈，其他菌株v3 ， v32有个别的交叉反应，但不强烈
Meq protein , highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro . the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef ) 利用通过杆状病毒载体在昆虫细胞系sfg上高度表达的meq蛋白产物免疫balb / c小鼠，然后收获其免疫脾细胞并与肿瘤细胞系spz / 0通过peg于体外融合;获得的杂交瘤细胞被克隆并通过与mdv感染的鸡胚成纤维细胞( cef )做免疫荧光试验( fa ) ，进行其分泌抗meq单克隆抗体( mcab )能力的筛选。
Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months . the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg , and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa . through three clonings less diversed anti - p24 hybridoma cells are gained 方法：基因工程p24抗原免疫小鼠4次，历时2个月，取脾细胞与骨髓瘤细胞株sp2 0 ，用peg融合， hat选择培养和间接elisa筛选分泌抗p24抗体阳性的杂交瘤细胞，三次克隆化后得稳定分泌抗p24抗体的杂交瘤细胞株。
