It was showed that rh - cm not only could maintain es cells in the undifferentiated state , inhibiting the spontaneous differentiation effectively , but also promote adherence and proliferation of mouse es cells 表明rh - cm能够促进es细胞贴壁增殖和es集落的形成，有效的维持es细胞未分化状态。
Callus 1 . a mass of undifferentiated parenchyma . if a plant is injured , the surrounding uninjured parenchyma cells form a suberin - impregnated callus , sometimes , sealing off the wound 愈伤组织： 1 .一团未分化的薄壁组织。如果植物受到损伤，那么周围未受伤的薄壁组织就会在伤口处形成具有分生能力的组织，有时可以封闭伤口。
Embryonic stem ( es ) cell lines isolated from primitive embryonic cells are highly undifferentiated and totipotent cells . in vitro , es cells can spontaneously differentiate into embryoid bodies ( ebs ) containing derivatives of three germ layers 胚胎干细胞( embryonicstemcell ， escell )系是从早期胚胎细胞分离培养而建立的高度未分化的具有全能性的细胞系。
Rather than injecting undifferentiated stem cells into damaged tissue , using precursor cells from a later stage of development - - ones already “ programmed ” to become retina or some other type of cell - - may be more successful , he says 不是通过注射未分化的干细胞进入损伤组织，使用来自发育后期的前体细胞（已经程序化成为视网膜或其他种类的细胞）可能更成功。
The results showed that es cells derived from kunming albino mouse remained undifferentiated state when they were cultured in vitro for a long time , and karyotypies were stable , but the ability of germ - transmission have not been verified 结果显示，用昆明白小鼠囊胚所建立的胚胎干细胞在体外长期培养时仍能保持未分化的状态，核型稳定，但还未证明其具有生殖系嵌合的能力。
At 48dah , the gonads of some southern catfish developed relatively slower than control group fish under the effect of fadrozole by the hislological dissection . meanwhile , the gonads of female fish had already appeared ovary cavity and oocyte 48dah组织学结果显示， fadrozole处理引起南方鲶一部分性腺发育迟缓，处于未分化状态，而此时的雌鱼性腺已经分化出明显的卵巢腔和卵母细胞。
The telomerase activity was decreased during differentiation the undifferentiated escs showed strongest telomerase activity . it decreased during differentiation . until on the tenth day , it was almost invisible , but the telomerase activity of the mef was still lower than that 3 .端粒酶活性随分化逐渐下降未分化escs端粒酶活性最强，随分化天数增加，端粒酶活性逐渐下降，至第10天，端粒酶活性已经很弱，但仍强于mef一p3 。
Escs can be induced into neuron - like cells by sequential neural induction the undifferentiated escs grew in colonies , with clear bound . alkaline phosphatase staining showed dark brown positive particles were distributed in every esc colony . after the sequential neural induction , the cells converted into neuron - like cells , with homogeneous forms , which had round bright cell bodies and thin long bipolar or multipolar processes 5诱导分化前后细胞端粒酶活性检测( trap )结果1 .使用序贯诱导法可将escs大比例诱导分化为神经元样细胞未分化的escs聚集成团状生长，细胞排列紧密，集落边界清楚，碱性磷酸酶染色呈强阳性，胞浆布满棕黑色颗粒。
The primary results showed : using m199 as diluents containing 20 % bovine serum , it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example , from 0 to - 6 freezing speed is about - 0 . 05 a minute , from - 6 to - 40 , freezing speed is about - 0 . 5 a minute ) , studies on effect of various concentration of dmso demonstrate that about 12 . 5 % dmso gave the highest post - thaw percentage of viable cells . the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus . the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula . the cells preserved in liquid nitrogen at - 196 were thawed and cultivated , a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 % . the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells 研究初步表明:以细胞培养液m199 (含2既的小牛血清，常规量双抗)为冻存稀释液对泥鳅胚胎细胞冷冻保存宜采取先慢后快的方式(例如，从0一一6 ，冻存速度为一0 . 05 / min ，再以一0 . 5 / min的速度从一6一一40 ) ; dmso的保护效应浓度为12 . 506左右;小牛血清的浓度对泥鳅胚胎细胞的成活率影响不明显;囊胚晚期细胞抗冻性比中早期强;通过对不同批次的冻存细胞解冻培养，解冻后成活率为30 %以上细胞培养数天后均有少数细胞贴壁，但只发现两瓶培养细胞有明显增殖现象产生许多未分化的小细胞。
This concern , recognized by a 10 - year - old , is very real : the potential of gamma - secretase as a therapeutic target is tempered by the fact that this enzyme plays a critical role in the maturation of undifferentiated precursor cells in various parts of the body , such as the stem cells in bone marrow that develop into red blood cells and lymphocytes 这个连10岁小孩都注意到的顾虑，是再实际不过的事了：虽然分泌酶可能是个治疗标的，但我们却不能不注意一个事实：这个酵素对身体各处未分化的前驱细胞（像是可分化成红血球与白血球骨髓干细胞）的成熟，扮演著关键角色。
