Because it is difficult to obtain antimicrobial peptides in large scales from animal tissues , the production of antimicrobial peptides by genetic engineering becomes the focus of people ' s interests 由于从动物组织难于获取大量天然抗菌肽，因此利用基因工程进行抗菌肽药物开发成为人们关注的焦点。
The result shows that the soluble part of human trail expressed by gtr48 have the native function of trail . antibacterial peptide is a kind of small peptide found in animals and plants and has a broad - spectrum antibacterial activity 抗菌肽是一种在动植物体内发现的一种具有广谱性抗菌作用的小分子多肽，它们在体内的免疫防卫系统中起到重要的作用。
The resistance of microbes to antimicrobial peptides can n ' t be formed because of their special mechanisms of killing the microbes . so the antimicrobial peptides are the most prospective substitutes for the antibiotics 抗菌肽由于其抑制或杀灭病原微生物的特殊机理消除了病原微生物易产生抗药性这一问题，从而最有希望成为补充和替代抗生素的新型药物。
Antimicrobial peptides are the immune response products of animals infected by the microbes . the antimicrobial pptides have many characters such as low molecular weight , thermostability , strong alkalinity and wide antibiogram 抗菌肽( antimicrobialpeptides )是多种动物对微生物等因素侵染产生的免疫应答反应的产物，具有分子量低、热稳定、强碱性和广谱抗菌等特点。
Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv , the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr 由于硫氧还蛋白和抗菌肽之间设计了肠激酶( enterokinase )切割位点和cnbr切割位点，通过对该表达的融合蛋白的切割，可得到目标抗菌肽cmiv突变体多肽分子。
Some antibacterial peptides such as pr - 39 , a member of cathelicidin family , can also be induced and expressed in liver as an acute - phase reactant . mouse - defensin - 3 ( mbd3 ) is only constitutively expressed in mucosal tissues 急性时相反应时，一些抗菌肽基因如cathelicidin家族中pr - 39等也能在肝脏中被诱导表达，作为一种急性时相反应因子参与组织修复和抗肿瘤免疫。
The artificial antibacterial peptide - ceme gene was designed according to the codon with the highest frequency in pichia pastoris , then the ceme gene was integrated into the chromosome of pichia pastoris strain - gsl 15 by electroporation 我们根据人工设计的新型抗菌肽ceme序列设计ceme基因，重组到酵母胞内表达型的整合载体phild2中，通过电转化作用将ceme基因整合至酵母宿主gsll5染色体上。
Cationic antibacterial peptides are found in insect , animal and plant . they are grouped into four classes including cecropins , magainins , melittins and defensins . cema consists of eight amino acid from n - terminus of cecropin a and c - terminus of modified melittin 阳离子抗菌肽是一类广泛存在于昆虫、动物、植物体内的小分子抗菌活性多肽，大体可分为：杀菌肽( cecropin ) 、蛙皮素( magainin ) 、蜂毒素( melittin ) 、防御素( defensin )等四大类。
It has been used to express in an attractive level a variety of intracellular and extracellular proteins of interest . ln our study , pichia pastoris gene expression system was used to express the soluble part of human trail ( tnf related apoptosis induced ligand ) and antibacterial peptide - ceme 因此它在基因工程方面的应用越来越受到人们的重视，已被广泛应用于一系列胞内及胞外蛋白的高水平表达。我们利用这个系统分别对人的trail ( tnfrelatedapoptosisinducedligand , trail acl2 )基因可溶性部分和抗菌肽ceme基因的表达进行了研究。
And the fusion protein could been purified quickly by thiobond ? resin . this was key step to produce cmiv largely by fermentation . meanwhile , the structure of the mutation ii of cmiv showed a new way to design peptide of cecropin which have the targeting sequence to tumor cell 以上研究的意义： 1 、初步解决了抗菌肽cmiv突变体在大肠杆菌中高效可溶性表达的问题，为进一步大量生产cmiv基因突变体打下基础； 2 、为进一步设计具有特异导向性的抗菌抗肿瘤cmiv突变体基因和表达以及以后进行动植物转化打下基础。
