Purification of the recombinant expressed endostatin in this work we have successfully cloned mouse endostatin gene , and constructed pbv220 - endostatin expression plasmid that is a non - fusion expression vector . the positive pbv220 - endostatin was transformed into dh5a , and expressed mouse endostatin protein successfully . this study is the basis of the endostatin gene - engineering production 本文成功克隆鼠内皮抑素基因，构建了内皮抑素基因的原核表达载体pbv220一endostatin ，该载体为非融合性表达载体，将pbv22o一endostatin成功转化到大肠杆菌表达菌dh5a中，并诱导表达了鼠内皮抑素蛋白，为基因工程方法生产内皮抑素奠定一定基础。
Endothelial cells were continuously cultured for 36 h after treating with angiostatin , endostatin and both of them , respectively . through transmission electron - microscope , we found all of them could induce apoptosis in endothelial cells . the effect of synergy between angiostatin and endostatin was stronger than either of angiostain or endostatin 本实验还探讨了人血管抑素可能的作用机理和人血管抑素、人内皮抑素以及两者的协同作用对体外培养的内皮细胞的增殖和对体内鸡胚新生血管形成的影响，并将三者的作用效果进行了比较。
So , by inhibiting the neovascularization to prevent the tumor growth and metastasis is a strategy for tumor therapy . endostatin was isolated firstly from the conditioned media of a non - metastatic murine hemagioendothelioma cell line eoma by o ' reilly in 1997 . endostatin is a 20kd c - terminal fragment of collagen xvh3 1997年o ' rilly首次从小鼠内皮细胞瘤培养液中分离出内皮抑素( endostatin ) ，氨基酸序列分析显示此蛋白是胶原c末端的氨基酸片段，分子量为20kd 。
