At first , 1 . 67 u g per well mcab all was coated on three wells of a plate , and then 1 . 5 x 1011 phage virion was diluted and added , after incubating with the target , wash away unbound phage by tbst ( 0 . 1 % tween - 20 ) , the bound phage was eluted with ph 2 . 2 tris - gly buffer and amplified , the specially bound phage was enriched by taking through addition binding / amplification cycles . ln the following cycles , the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all , collecting and titering the washing phage of last time and output phage in each round , the selective ratio and the false positive rate of each round were worked out , the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective . after 4 rounds of panning , 11 phage clones were selected after competitive - ellsa , the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced , a clear consensus binding sequence emerged 在本实验中，利用随机12肽库对抗猪瘟病毒( classicalswinefeverviruscsfv )糖蛋白me2的单抗a11进行表位筛选，经过四轮筛选以后，随机挑取11个克隆作竞争- elisa检测，结果表明，所挑11个克隆中，有9个克隆能对me2蛋白和a11反应产生抑制作用，抑制率最高可达64 ； dna测序以后经过dnastar软件分析，发现它们的核心序列为anwralsl ，该核心序列与猪瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夹心- elisa检测和western - blotting试验均证明所挑阳性克隆能被a11所识别；人工合成含核心序列的多肽经间接elisa试验证实，也能被a11识别。
The results showed that the extracts from the whole of solanum nigrum had 100 % inhibiting rates to rhizoctonia solani and cladosporium ucumerinum fruits of solanum nigrum also had 100 % inhibiting rates to fusarium oxysporium , extracts from fruit of xanthium sibiricum had 100 % inhibiting rates to rhizoctonia solani ; extracts from the whole of artemisia annua had more than 78 . 5 % inhibiting rates to fusarium oxysporium , alternaria solani and rhizoctonia solani 结果表明：龙葵全株粗提物对辣椒丝核和黄瓜黑星病菌、龙葵果实提取物对黄瓜枯萎病菌、苍耳果实提取物对辣椒丝核病菌的抑制率均为100 % ；黄花蒿(全株)提取物对黄瓜枯萎病菌、番茄早疫病菌和辣椒丝核病菌的菌丝抑制率都分别达到了78 . 5 %以上。
The animal study was designed to determine whether ph - egf fusion protein was protective to ethanol induced injury of gastric mucosa in rats . thirty - two sd rats weighting 200 - 250g starved for 24hour were divided into four groups which received pupae infected with recombinant bmbacph - egf virus , normal pupae , ranitidine , 0 . 9 % saline respectively for 3days before received absolute ethanol . the results showed that both pupae infected with bmbacph - egf virus and normal pupae protect the gastric mucosa against ethanol induced damage 本研究还建立了动物模型，大鼠乙醇灌胃后的胃粘膜经病理学观察和病理组织学检查，发现重组病毒感染蚕蛹5天的样品灌胃后对乙醇诱导溃疡的预防性抑制率达50左右，但正常蚕蛹样品也有一定的预防乙醇诱导损伤、保护胃粘膜作用。
For the acute exposure duration , there expressed the dose - thresholds and time - thresholds in the effects of cu2 + on the activities of cat , pod and sod in two macrophytes . when the test species were exposed at low dose and for a short period , cu2 + can stimulate activities of antioxidant enzymes . once exceeding the thresholds , cu2 + may inhibit enzymes activities Cu ~ ( 2 + )低浓度和短时间内对抗氧化酶有诱导作用，超过一定的阈限会产生抑制作用；金鱼藻cat 、 pod 、 sod活性在7天内的最大抑制率与暴露cu ~ ( 2 + )浓度呈显著正相关。
