All samples of the tumor tissues were observed by electron microscope . the t lymphocyte subsets and b lymphocytes were detected and counted with monoclonal antibody technique before and after the cryotherapy . results : the cured percentage of internal hemorrhoid , mixed hemorrhoid and chronic fissura ani reached 83 . 5 % , 66 . 8 % and 62 . 4 % respectively . the clearance rate of the verruca acuminata was 100 % 方法：取自西京医院15年间冷冻治疗肛肠区良性病变7018例，恶性病变636例，冷冻前后经电镜观察癌细胞变化及应用单克隆技术检测患者t细胞亚群、 b细胞水平。
