The so - called transgenic insects would be resistant to infection by falciparum . through breeding , the altered insects would gradually replace the population of mosquitoes capable of becoming infected with the parasite 这种所谓的经过基因改造的昆虫对恶性疟原虫有抵抗性。通过繁殖，这些蚊子可以取代可能被寄生虫感染的蚊子。
The so - called transgenic insects would be resistant to infection by falciparum . through breeding , the altered insects would gradually replace the population of mosquitoes capable of becoming infected with the parasite 这种所谓的转基因昆虫可以抵抗恶性疟原虫的感染。通过培养，这种转基因昆虫将会逐渐取代原来会被寄生虫感染的蚊子。
The so - called transgenic insects would be resistant to infection by falciparum . through breeding , the altered insects would gradually replace the population of mosquitoes capable of becoming infected with the parasite 这种被称做“转基因昆虫”的蚊子可以抵抗恶性疟原虫的传染。通过繁殖， “转基因昆虫”会逐代取代会感染疟原虫的蚊子群体。
Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using . triplix kit . a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction . the ss - dna was reversely transcripted from total rna . double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i , the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro . a high titer and high recombinant ratio of cdna library was constructed 构建恶性疟原虫海南株红内期cdna表达文库提取红内期恶性疟原虫海南株总rna ，直接以总rna为模板使用cdna文库构建试剂盒，首先反转录合成ss一dna ，再扩增合成ds一dna ( cdna ) ，对扩增产物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶试剂盒纯化、回收20obp以上的片断，经与载体连接再用蛋白包装物包装后形成未扩增文库，最后扩增完成恶性疟原虫海南株红内期cdna表达文库的构建。
We screened the cdna expression library by using 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether conjugated with bsa . one of the screened positive clones was inserted tctp of plasmodium falciparum from hainan . the tctp was cloned to express protein in order to provide materials for further study 把青篙素类衍生物: 12b一二氢青篙素( 4 ’一氧乙酸基)苯醚用bsa标记作为探针，对恶性疟原虫海南株cdna表达文库筛选，分离出插入了恶性疟原虫海南株tctp基因的阳性单克隆。
