Phaa , phab and phac were amplifed from the subclone of pseudomanas sp . producing pha by pcr . the gel electrophoresis analysis showed that the molecular weights of cloned phaa , phab and phac were equal to fragment speculated from three orfs 利用所提取的开放阅读框架的序列设计三对引物，采用pcr技术，从合成pha的亚克隆片段中分离出phaa 、 phab和phac三个基因片段，经凝胶电泳分析表明，所克隆的三个基因分子量大小与推测的三个开放阅读框架中基因片段大小一致。
However , only a few host factors with clear in vivo function have been identified . by using pcr and 5 " and 3 " race , we were able to clone a homologue ( named ttom1 ) of arabdopsis thaliania host factor gene , tom1 , which supports the replication of tobacco mosaic virus 根据从拟南芥中已经克隆的支持烟草花叶病毒复制的基因tom1序列设计一对特异性引物，用rt - pcr的方法获得番茄同源基因的部分序列，然后利用5 ’ race与3 ’ race方法从番茄中克隆出全长ttom1基因。
The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants . in this study , full length of phrip1 is amplified by pcr and ligated into pks plasmid , then the bait plasmid , peg202 - phrip1 , is constructed . the inseret gene are sure to be translated into the right fusion protein through its sequence . in the yeast two - hybrid system , the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation . then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained . the two insert gene fragments are sequenced . one of them is plastocyanin , the other is putative photosystem i reaction center subunit ii precursor , both of them are the necessary components of photosynthetic chain 成膜素相关蛋白1 ( phrip1 )是一个含608个氨基酸的蛋白质，它对于植物胞质分裂中细胞板的形成起到了十分重要的作用。研究phrip1的功能和机制，对在分子水平上阐明植物细胞板以及细胞壁形成的机理具有重大的生物学意义。在本实验中，根据phrip1的序列设计引物对其进行pcr扩增，得到该基因后将其连接到了pks质粒上，并进一步构建成了诱饵质粒peg202 - phrip1 。
The full coding regions of bdnf genes were amplified from the genome of tylototriton taliangensis , phrynocephalus hongyuanensis , japalura splendida and cyclophiops major , respectively by pcr with the primers designed on the sequence of human bdnf gene . the pcr products were cloned into the vector pucis of esherichia coli . sequence analysis showed that the coding regions of three reptiles are the same ( 741 bp ) in length and these bdnf genes encode a peptide of 246 amino acid residues while that of tylototriton taliangensis is 744 bp in length and encodes a peptide of 247 amino acid residues 根据已有的人bdnf结构基因的全长序列设计了一对引物，利用pcr技术分别从大凉疣螈( tylototritontaliangensis ) 、红原沙蜥( phrynocephalushongyuanensis ) 、丽纹龙蜥( japalurasplendida )和翠青蛇( cyclophiopsmajor )的基因组dna中扩增到目的dna片段，并将其分别克隆到大肠杆菌载体中，然后对所获得的阳性克隆进行测序。
This dissertation , based on the theories of nonlinear dynamics and modem communications , and with an aim to apply the excellent chaos characteristics to the communication system to efficiently improve its performance , focuses its study on chaotic correlation synchronization , enhanced fm - dcsk modulation , optimal chaotic sequences designing , vd - rake receiver and arithmetic separating of chaotic signals and noise 本论文紧紧围绕混沌通信这一主题，以非线性系统理论和现代通信理论为基础，将混沌优良的特性应用于通信系统中，重点研究混沌通信中混沌相关同步、改进的fm - dcsk调制方式、最佳混沌序列设计、 vd - rake接收机以及基于流形分解的混沌信号与噪声的分离算法等关键问题。
The principles of erasure codes used under binary erasure channels are summarized and erasure codes which belong to standard classes of rs codes are introduced with emphasis on cascaded low - density erasure codes with linear time encoding and erasure recover algorithms . thresholds of regular degree distributions are analyzed . it is shown that low - density erasure codes based on ( d , 2d ) - regular sequences of degree distribution are not close to optimal ( d 3 ) . two pares of irregular degree distribution sequences are introduced and a pare of improved right regular sequences of low - density erasure codes are presented , it is testified that the new sequences are asymptotically quasi - optimal . in the meantime , simulations of cascaded low - density erasure codes based on a few types of special sequences of degree distribution available are given , together with performance analyses on these codes 阐述了应用于删除信道下的纠删码基本原理，介绍了两类标准的rs码类纠删码，重点分析了具有线性时间编码和恢复算法的渐近好码?级联型低密度纠删码，分析了正则度分布的阈值，对正则低密度校验码在删除信道下的纠错性能进行了仿真，从理论上证明了基于( d ， 2d ) -正则度序列的低密度纠删码都不是渐近最优码( d 3 ) ，同时还分析了非正则低密度校验码的度序列设计，基于右边正则序列提出了一种改进型右边正则序列，证明了此序列为渐近拟最优的，对基于几类现有典型度分布序列的级联型低密度纠删码进行了模拟仿真及性能分析； 3
Chapter 2 cloning and analysis of the full - length cdna of ejo1 gene related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) primers were designed according to the sequence of est004 ( dbest accen - ssion number : ca591895 ) which was gotten using ssh and cdna macroarray approach 3中华城螫蟹卵巢发育相关基因ej03的全长0 a克隆和序列分析根据ssh结合cdna微阵列技术获得的est003 （ dbest检索号： ca591894 ）的序列设计引物，用5 ’ race和3 ’ race的方法获得了ejo3基因的全长cdna序列。
Sequencing of the whole alpha - amylase gene of bombyx mandarina ( chongqing ) based on the published nucleotide sequence of alpha - amylase gene of bombyx mori , seven pairs of primers were designed for the sectional amplification and cloning of the alpha - amylase gene of bombyx mandarina . the combined sequence , i . e . the whole alpha - amylase gene of bombyx mandarina , is 8903bp in length 重庆野桑蚕-淀粉酶基因全序列的测定根据家蚕-淀粉酶全基因序列设计了的7对引物，对野桑蚕-淀粉酶基因进行了分段扩增，并逐一克隆测序和序列拼接，得到8093bp的野桑蚕-淀粉酶基因全序列。
Besides , vgb gene expression also increased the chitinase secretion . in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis , an inducible promoter ( levansucrase ) was cloned from b . subtilis wb600 and ligated with a promoter - less vgb gene . the resulted gene is called sacvgb and was demonstrated to express in e , coli by sds - page and carbon monoxide binding assay 由于vgb基因启动子不能在枯草杆菌中启动表达，因此，根据已发表的果聚糖蔗糖酶基因( sacb )序列设计引物，从枯草杆菌wb600总dna中扩增出该基因的启动子片段，然后将其与vgb基因编码区及终止子序列相连，成功地组建了sacvgb融合基因。
According to the amino acid sequence resulted from our previous research about tb22kda protein and the related literatures about gene sequence of allergenic protein in common buckwheat , we designed primers and got the structure gene successfully . by 3 ' - race method combined with nested pcr , the 3 ' - end nuclear acid sequence was also obtained ; in additon , for the 5 ' - end sequence , we selected a specific conserved nuclear acid sequence as the 5 ' primer and part of structure gene sequence as the 3 " primer , and till now , partial 5 ' - end sequence has been amplified as well 本研究根据先前分离纯化所得天然tb22kda蛋白经maldi - tof - ms (质谱法)测得的氨基酸序列和文献报道的过敏蛋白核苷酸序列设计引物，扩增克隆了该过敏蛋白结构基因的编码序列；根据测得的序列设计特异性引物，并利用3 ’ - race方法结合巢式pcr扩增得到基因的3 ’末端；依据同源性比较的结果选用一段保守序列为5 ’引物，并根据结构基因内部序列设计3 ’特异性引物，进一步获得了该基因5 ’端的部分序列。
