However , it is a key problem with dna microarrays how to generate higher fluorescent signals to improve the detection sensitivity 对寡核苷酸芯片而言，仍有待解决的问题是如何产生更强的荧光信号来改善其检测灵敏度。
Oligonucleotide microarray technology is a powerful data - mining platform and has been widely applied in biosciences 摘要寡核苷酸芯片技术是一种高通量发掘和采集生物信息的强大技术平合，目前已广泛应用于生物科学领域。
Inhibition of the activation and collagen production of cultured rat hepatic stellate cells by antisense oligonucleotides against transforming growth factor 1反义寡核苷酸对大鼠肝脏星状细胞激活及其胶原生成的抑制作用
Inhibitory effects of antisense oligonucleotides targeting mitogen - activated protein kinase mapk mrna on neonatal rat cardiac fibroblast proliferation induced by ang and egf 反义mapk寡核苷酸对ang及egf诱导的心肌成纤维细胞增殖的抑制效应
Oligomap - oligomap is a tool to help researchers engineer restriction sites into oligonucleotides using silent mutagenesis and reverse translation algorithms Oligomap是一个帮助研究人员使用哑诱变和反向转换算法来约束寡核苷酸位置点的工具。
Two types of multiply labeled primers , termed multiply labeled linear primer and multiply labeled branched primer , were used to enhance the fluorescent signal obtained from two - dimensional dna microarrays 利用两种类型的多个荧光分子标记的引物，来增强二维寡核苷酸芯片平面上的荧光信号强度。
Prepare the oligonucleotide probes designed 16 probes as four groups according to hla - dqa1 sequence in genbank and modify each probes by ammonia at 5 ' - end . then resuspended the lyophilized probes in ph = 9 , 0 寡核苷酸探针的制备根据genbank数据库新近公布的hla - dqa1等位基因序列，设计四组共16条特异性寡核苷酸分型探针，并在各5 ’端做氨基修饰。
Comparisons were performed by using dna microarrays containing 16 , 228 65 - mer oligonucleotides representing zebrafish genes and ests . rna was isolated from the pooled digestive tracts of 30 animals per treatment group 利用dna芯片，包含16 , 228个65 -碱基对寡核苷酸，代表斑马鱼基因和表现序列标志（ est ） ，来进行比较。每个实验组有30只动物，从其消化道中分离出rna 。
Id like to fabricate the hla genotyping chip by the method developed in our lab , and typing hla allele in dqa1 . i am in an attempt to establish a mature technique process to typing the complicated hla system 本研究使用本实验室建立的技术流程制作寡核苷酸芯片，对hla - dqa1位点进行等位基因分型检测，旨在建立一套成熟的寡核苷酸芯片分型技术，用于复杂的hla系统的分型研究。
To improve the performance of assays on the oligonucleotide microarray , the factors that influence the hybridization effects such as surface chemistry , probe size , spacer length , hybridization conditions etc were intensely studied and optimized 为改善寡核苷酸芯片的分析性能，对影响芯片杂交结果的因素，如片基表面的化学处理、探针的长度、间隔臂的长度、杂交条件等，进行了深入的研究和优化。
