The system is also amenable to genetic transfections , and provides a very short incubation period for studying the role of various genes 此外，这套实验系统同时能够进行基因转移感染实验，并可在很短的培育时间下研究各种基因角色。
Additionally , baculovirus has been a new gene transfer vector used in gene therapy due to its advantages of the extremely high expression of foreign genes and being non - reproduce vector 另外，杆状病毒是非复制型载体，能高效表达目的蛋白，其作为基因转移载体在基因治疗中乙显示出良好的应用前景。
Kurane s , kranss jc , watari e , et al . targeted gene transfer for adenocarcinoma using a combination of tumor - specific antibody and tissue - specific promoter [ j ] . jpn j cancer res , 1998 , 89 ( 11 ) : 1212 李芳,伍新尧,郭语彬,等.阳离子脂质体介导肺癌细胞基因转移效率的比较研究[ j ] .中山医科大学学报, 2000 , 21 ( 6 ) : 484
Among a gradient of injected plasmid dna mass , which was from 250ng to 1850ng , the 1850ng smi - created prawn embryos showed a gene transfer rate of at least 16 % 从250ng到1850ngpcmv一sbwafp一sv40utr质粒dna的注射剂蛋范围内，都可得到有整合的转基因胚胎。注射185ong质粒dna获得了至少16 %的基因转移率。
The students said on tuesday that they have genetically modified a plant using a green fluorescent marker gene from jellyfish , so that it " lights up " when it is stressed as a result of dehydration 具体做法是将一种从水母体内提取的可令物体发出绿色荧光基因转移到该植物内。这样植物在缺水“口渴”时会立即变得“闪闪发光” 。
Winston - salem , n . c . - - the first human study using gene transfer to treat erectile dysfunction ( ed ) shows promising results and suggests the potential for using the technology to treat overactive bladder , irritable bowel syndrome and asthma , according to the researchers 研究者称，第一个用基因转移方法治疗勃起功能障碍（ ed ）的人体实验显示有良好结果，这种方法同样具有治疗过度反应的膀胱，过敏性肠综合征和哮喘的潜力。
Recent studies show that dna transfer and recombination technology provides great potential for genetic improvement of zoysiagrasses ; molecular linkage maps were constructed from zoysia japonica and its hybrids with zoysia matrella ; the researches on gene cloning and gene resource are in progress 最近的研究表明，基因转移与重组技术在结缕草遗传改良中具有巨大应用潜力；结缕草遗传连锁图谱构建取得重要进展；基因克隆和基因资源研究正在展开。
This paper reviews the methods for genetic transformation used in rapeseed , the genes introduced into rapeseed , transgenic rapeseed lines permitted to carry out field trials in china , transgenic rapeseed cultivars developed and released abroad , as well as canadian practice of planting herbicides - tolerant rapeseed cultivars 摘要介绍了油菜基因转移的方法，已转移到甘蓝型油菜中的基因，我国已进入中间试验的转基因油菜品系，国外已育成的油菜转基因品种，加拿大种植抗除草剂转基因油菜品种的实践等。
The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action , thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study , we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons 本研究设计以c tamrna为模板的反义cdna片段，从c ta基因5 ’端第94位到500位核苷酸段设计引物，目的片段407bp ，覆盖第116和188位两个aug密码子，也包含了第一外显子和第二外显子间的剪接位点：用常规分子生物学方法构建了反义片段的腺病毒表达载体( padeasy - 1系统) ；腺病毒载体经hek293细胞包装产生含反义片段的重组腺病毒，用氯化铯密度梯度离心法获得纯化的高滴度腺病毒；进行体外基因转移，分别用反义片段真核表达载体转染p388d1细胞和用重组腺病毒感染hela细胞，观察导入的c ta基因反义rna抑制细胞内组成型或诱导型c ta基因表达的作用，从而达到调控mhc -类分子表达的目的。
Dr jacques diouf , director - general of the un food and agriculture organisation , stated at the release of sofa 2004 " we will have to use the scientific tools of molecular biology , in particular the identification of molecular markers , genetic mapping and gene transfer for more effective plant enhancement , going beyond the phenotype - based method " 中提出“我们将会必须使用分子生物学的科学工具，特别是在分子记号，遗传定位和基因转移的确认来做比较有效的植物增强，去超过以显型为基础的方法。
