It is very important to investigate and clone related genes under fe - deficiency stress in malus xiaojinensis , which not only provides the probability for exploring molecular mechanism of the absorbability and transport of fe , but also may enhance the resistance under fe - deficiency stress of plants by inserting related genes into chromosome genome 因而研究和克隆缺铁胁迫相关基因不仅为进一步研究苹果吸收、转运铁的分子机制提供基础。其次，在提高植物对铁胁迫抗性的遗传育种中，可以利用基因工程技术，将铁胁迫相关基因整合到作物染色体基因组中，从而达到有效控制铁营养的目的。
Acetylornithine deacetylase is the key enzyme of producting l - methionine . we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase . in order to get high expression deacetylase strain , we obtain the gene by pcr arge gene . the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr . then taking the nucleotide sequencing compared with the sequence at blast of u . s . a . we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g 为了获得高效表达的脱乙酰鸟氨酸酶工程菌株，在工程菌技术改造及其固定化研究做了进一步的研究和探讨。我们采用基因工程技术，通过pcr技术扩增出了酰化酶关键酶基因?脱乙酰鸟氨酸酶基因arge ，将其克隆到puc19载体中，经酶切鉴定、 pcr鉴定筛选出重组阳性质粒，并测序鉴定，通过美国blast程序进行了基因数据库相似性比较分析。
Conclusion : we have established a kind of high density fermentation technology pattern and established a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies . at present , producing recombinant proteins with genetic engineering technology have gotten extensive development in our country , this program explored out a practical feasible procedure for the lower reaches of genetic engineering fermentation and purification technology 目前，利用基因工程技术生产目的蛋白已在我国得到广泛发展，对于重组产物而言其下游工艺尤其重要，本课题为基因工程下游发酵、纯化工艺路线探索出一个切实可行的程序，同时也为基因工程产品产业化放大提供了一个有益的借鉴。
In order to break down the rate - limited steps in the artemisinin biosynthesis to improve the artemisinin production and realize the industrial production of artemisinin , related key genes in artemisinin biosynthesis must be cloned and the regulatory patterns of key genes should be studied . for this purpose molecular cloning of related key genes in artemisinin biosynthesis was performed in this thesis work 利用现代分子生物学和基因工程技术手段，克隆青蒿素生成途径的关键酶基因，研究关键酶基因对青蒿素生物合成的调控规律，是打破青蒿素生物合成的限速步骤，大幅度提高青蒿素含量，最终达到利用植物生物技术工业化生产青蒿素的目的必须解决的关键问题。
Reviewing the usage of the biodegradation of refractory organic pollutants , including the biodegradation activities under co - substrates conditions , the application of anaerobic - aerobic treatment process , the usage of immobilized microbial cells technology , the appliance of membrane biological reactor and the usage of genetic engineering technology , the principles and advances of these technologies were discussed 摘要从共基质条件下的微生物降解处理、厌氧好氧发酵工艺、固定化生物催化剂技术、膜生物反应器以及基因工程技术等方面综述处理难降解有机污染物的降解原理及研究进展。
In the part two , gene engineering and life ethics . this part is written that the operation of gene engineering can harm the rights of people , including gene diagnosis make people privite lose and make the public discrimination , gene therapy can make people longevity , bue it also can make the living not sense , trans - foodstuff is secure or not , and should let know the details and choose themsleves when it is on sell . in the part three , the prevention to heredity disease and the ethical problems 在第二章基因工程与生命伦理中，主要分析了基因工程技术的应用给人类基本权利的维护带来的冲击，包括基因诊断会导致人隐私权的丧失和公众的歧视；基因治疗导致的长生不老会贬损生命的价值和意义；转基因食品是否会影响人体健康，转基因食品的销售应维护公众知情选择权等等。第三章主要论述了遗传病的基因预防和代际伦理。
