The first member be found ofndrg gene family is ndrgl , which was isolated as a new gene upregulated in n - myc mutant mouse embryos and ft is repressed by n - myc and c - myc Ndrg基因家族中最早被发现的是ndrg1 ,它是在研究n - myc下游调节基因时从小鼠胚胎中发现的一种新基因。
Objective to analyze the differences of n - acetylgalactosaminyltransferases gene expression profile in different human tumor cells with preliminarily made cdna microarrays 目的本研究初步创建了低密度基因芯片,并对人不同肿瘤细胞中的n -乙酰氨基半乳糖转移酶基因家族进行表达谱分析。
In this study , map2 and sap2 , two homologs ofap2 in arabidopsis and hap2 in hyacinthus orientalis l . , were isolated from the flower buds of apple ( mains pumila mill . ) 拟南芥的ap2和ant基因、玉米的gl15 、 ids1基因、矮牵牛的phap2a 、 phap2b基因以及风信子的hap2基因都属于这一基因家族。
The blast analysis of the 3 . 0kb segment indicate that it is similar to translational activator and has more than 80 % homology to two genes in arabidopsis and oryza saliva respectively 将所得约2 . 8kb的基因片段作blast分析,表明其可能属于翻译激活子基因家族,并在拟南芥和水稻中皆有同源性大于80的同源基因。
The coding sequences for the ntfs were then cloned into the expression vector pgex - 4t - 3 under the control of a tac promoter , and expressed in escherichia coli after induction with eptg 本论文经引物设计与合成后,利用pcr技术分别自大熊猫和朱鹤的基因组中克隆了gdnf基因和nts基因家族各成员成熟肽的完整编码序列。
We therefore also performed allelism tests between ll and as mutants . our results show that ll1 is the as2 mutant , and ll2 is the as1 mutant . i have cloned the as1 gene by map - based cloning 用图位克隆的方法,克隆了影响拟南芥叶极性发育的重要基因as1 ,基因序列分析表明, as1属于myb基因家族,可能是一种转录调控因子。
We cloned two ent - kaurene oxidase homologues from the reverse transcriptional products of different strawberry tissue , the identity of these two homologues is 88 . 2 % . it suggest us that it belongs to a gene family . 3 在丰香草莓的不同发育部位克隆到同源性为88 . 2的两个贝壳杉烯氧化酶的基因片段,预示着此基因是个基因家族。
As a considerably large amount of dna sequences become increasingly available , they are widely used to study phylogeny of species and multigene families , as well as the mechanism of evolution at molecular level 基于相当大量的dna序列的获得,其被广泛地用于物种的系统发生、多基因家族的演化,以及在分子水平上的进化机制的研究。
At the same time , it also preliminarily proves that there is a certain relation between n - acetylgalactosaminyltransferases gene expression levels and tumor formation , which necessitates more detailed studies 同时也初步证明: n -乙酰氨基半乳糖转移酶基因家族表达和肿瘤的发生有着一定的相关性,但它们之间具体的联系还需进一步的研究。
Two rnai constructs were made to validate the function of osdd2 gene . one contained the conserved wrky domain and the other did not . at present , we obtained many transgenic rice lines with one of the plasmids 同时为了避免可能存在的对wrky基因家族中的其它成员也带来影响,又构建了不含保守域的rnai载体,目前反向重复的干涉结构已经完成。