Westen immunoblotting with dl antibody and an approach detecting thylakiod phosphorylated proteins were used to check dl proteins of different forms , quantitive scranner to count abundances of proteins and fluorometry to detect the maxmin fluorescence ( fm ) of chlorophylls and influorescence of proteins 实验结果显示： ( 1 )光抑制处理使叶绿素a和叶绿素b发射荧光明显下降； ( 2 )光抑制处理时类囊体膜蛋白内源荧光并不发生明显变化。
This enzyme was different with the ones reported in the past . a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica , by nacl extration , ammonium sulfate precipitation , ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column 使用nacl抽提、硫酸铵分步沉淀、离子交换和butyl - toyopearl650m疏水柱层析等方法，从蕹菜叶绿体类囊体膜中分离纯化到一种蛋白磷酸酯酶。
From above , we indicate that : photoinhibition cause chlorophyll an " bleak " while influorecsence of proteins on thylakiod membrances has no prominent response to it . what ' s more , photoinhibiton induce d1 protein phosphorylated and aggregated , forming the aggregators with phosphorylated d1 结果表明：光抑制对叶绿素荧光具有漂白作用，对类囊体膜蛋白内源荧光无明显影响；光抑制处理引起d1蛋白磷酸化并发生聚合，且聚合物中d1蛋白以磷酸化形式存在。
They are involved both in the production of mature proteins , acting as processing enzymes , and in the degradation of damaged or non - functional proteins [ 1 " 8l in plant plastids , proteases function in protein degradation seem to act to adjust the stoichiometry of subunits in supramolecular complexes , as well as to regulate the stoichiometry between different supramolecular complexes in response to environmental changes and process the nuclear - encoded preproteins in the stromal 除了在相关蛋白质转录和翻译方面的调控外，专一降解这些蛋白的蛋白酶活性以及转录翻译量的上升也会加强对这些蛋白质的降解。虽然到目前为止对光系统中蛋白酶的了解尚且不如对光系统中各种多肽以及蛋白色素复合体的了解，但是对类囊体和叶绿体中蛋白酶的研究已经进展到相当深度了。
3 : chlorophyll content : low - temperature can restrain the biosynthsis of chlorophyll and destroy its structure . the contents of chlorophyll a , chlorophyll b and chlorophyll a + b in controls were significantly lower than in transgenic seedlings . the ratio of chlorophyll a / b had the similar result 低温处理后，对照中叶绿素a 、叶绿素b 、叶绿素a + b总含量均显著低于转基因苗，叶绿素a b的比值反映叶绿体中类囊体膜的垛叠程度，对照的叶绿素a b的比值低于转基因苗。
Recovery of this photoinhibition is a complicate but orderly course , including degradation of photodamaged d1 , synthesis and assembly of new one , etc . using lincomycin to block the replacement of new synthetic dl protein into photodamaged one , the spinach leaves was exposed to highlight , giving rise to photoinhibition before the thylakiod membranes were isolated 解除光抑制后， ps活性恢复是一个复杂而有序的过程，需要d1蛋白降解、新合成d1蛋白和重组装ps等。实验首先进行菠菜叶片光抑制处理，加入林可霉素阻断叶绿体蛋白质合成，利用尿素sds变性电泳分离类囊体膜蛋白，借助d1蛋白抗体westen免疫印迹、磷酸化蛋白快速检测方法分析d1蛋白存在形式，并进行定量分析。
At low concentration of zn , the changes in ultrastructure were nuclei deformation , chloroplast swelling and disorder of thylakoid arrangement ; serious damages in ultrastructure caused by greater zn stress were indicated by scattered nucleoli , condensed chromatin , almost empty nuclei with nuclear membrane disrupted and nucleoplasm flowing into cytoplasm , swollen and partly dissolved cristae of mitochondria , disrupted and collapsed chloroplast envelopes , and some dissolved thylakoids that flew into cytoplasm 超微结构的变化也呈现加重趋势，低浓度处理的变化为细胞核变形、叶绿体膨胀、类囊体排列紊乱；严重的超微结构的损伤是核仁散开、染色质凝集，细胞核几乎成为空核和核膜破裂，核质散出；线粒体脊突膨胀和部分溶解；叶绿体膜断裂、消失和部分类囊体溶解和散到细胞质中。
For uncovering the effects of reversible phospharylation on the structure and function of psii reaction centre , we purified a protein phosphatase associated membrane from the thykaloid membrane of ipomoea aquatica chloroplasts . in our experiments we studied the enzymology and spect rum characters of the purified phosphatase . in our lab , one kind of protein phosphatase associated thylakoid membrane of pomoea aquatica has been isolated 迄今人们对类囊体蛋白磷酸酯酶的研究较少，为了研究可逆磷酸化对psii反应中心结构与功能的影响，本文以蕹菜为材料，从叶绿体类囊体膜中分离纯化到一种膜结合蛋白磷酸酯酶，进行了酶学性质和光谱性质的研究。
There is no thumb - shaped area on the back of the compound eye . the results under transmission electron microscope show that the fine structure of the compound eye in portunus tritubereulatus is different from that in metapenaeus ensis . the former has eleven retinular cells which composed the compound eye 两部分的小网膜细胞在结构上基本相同，都含有线粒体、多囊体、内质网、色素颗粒等，但是上部的小网膜细胞没有扳膜体，而且胞质比较致密，膜下储泡囊的空腔较小，里面有膜状结构。
Chloroplast phosphoprotein were first found in thylakoid membranes by bennett o the attachment or removal of a phosphate group from a protein may have profound effects on that protein ' s activities and properties , the reversible phospharylation of the membrane protein in thykaloid is a dymanic equilibrium process 而bennett ( 1977 )发现的类囊体膜蛋白可逆磷酸化已成为近年研究的热点之一，蛋白质连上或是移去磷酸基团都将强烈影响蛋白质的生理活性和性质，这需要类囊体膜蛋白激酶和磷酸酯酶的参与。
