After stimulation of insulin > epinephrine in 10 ~ 6m to the differentiated 3t3 - l1 cells for 48h , or incubated with 16 . 8mm glucose for 48h , the crude membrane proteins were extracted , then investigated the effects of various factors on aqpap protein expression by western blotting . the subjects were divided into three groups according to body mass index : the normal , the overweight , the obese . the expression of adipose aqpap were detected with rt - pcr and western blotting 2 、利用半定量rt - pcr及westernblotting技术，从基因及蛋白质表达水平研究激素(胰岛素、肾上腺素、地塞米松、 igf - 1 ) 、营养物质(葡萄糖、甘油) 、调脂药(非诺贝特、洛伐他汀)对3t3 ? l1脂肪细胞aqpap表达的影响，并观察不同体重研究对象脂肪组织aqpap表达量的变化。
The fracture energy of cu / - aiodiffusion - welded joints was greatly increased by the introduction of the thin film nb interlayer whereas diffusion welding can be performed at a relatively low temperature ( 900 ) . the increase in fracture energy of the joints with nb film interlayer was attributed to the strong abhesion of nb to - aio combined with larger plastic deformation in the metal side during fracture tem observations showed large amounts of dislocations existing at the interfacial regions 本文主要内容就是概括地介绍了国内外关于扩散连接接头行为数值模拟的发展现状；主要包括界面孔洞消失过程、接头元素扩散与反应层的形成、接头变形与应力行为的数值模拟，以使人们能够定性或半定量的分析扩散连接因素对接头性能的影响。
The average species similarity was 0 . 68 between semi - quantitative ( kick sampling plus d - frame net sampling ) and quantitative samples in the same site , higher than that of 0 . 56 in the lotic water , 0 . 45 in the lentic water . 3 . no significant difference was found in metrics calculated from the data of quantitative and semi - quantitative samples in the same site respectively 从种类相似性看，同一样点半定量样本(踢网样加d形抄网样)与定量样本(索伯网样)之间的相似性最高，平均为0 . 68 ，高于急流栖境中定量样与半定量样的种类相似性0 . 56 ，以及静水-缓流栖境中，定量样与半定量样的种类相似性0 . 45 。
I a study on the comparison between quantitative and semi - quantitative sampling methods for stream macro - invertebrate assemblages quantitative sampling method for stream macro - invertebrates is well used in stream ecological study , and semi - quantitative method is widely used in water quality bio - assessment . the results from comparisons of the number of species , individuals and values of metrics between the two methods were showed as follows : 1 对定量采样法和半定量采样法在个体数、种类数和种类相似性及生物指数等方面关系的研究，为探讨半定量采样法的科学性和实用性，进一步完善水质快速生物评价采样法(半定量采样法)提供科学数据。
Aberration of electronic lens made by electron gun and aberration of magnetic deflection system made by dy are comprehensively investigated , so is the shadow mask ' s effect on electron beam landing screen error . the conclusion can be get that , because the distribution of electron beam landing screen ( distribution of luminance ) is affected by many kinds of factors , it cannot get the correct function by calculation , and should be get by measurement instead 全面分析了cpt电子枪发射系统形成的电子透镜像差与磁偏转系统形成的偏转像差；分析了荫罩的自身厚度与位移对电子束着屏的影响，并由此得出结论，着屏电子束分布(即亮度分布)受着许多因素的影响，理论分析是半定量的，着屏电子束分布需要用精确的测量仪器来测量。
