Results also showed that lectins such as pha did not have distinct influence on increasing of metaphase of scallop . intergeneric hybridization between patinopecten yessoensis and chlamys farreri yielded hybrids which can survive to adult 本论文还对pha等外源凝集素对扇贝成体组织染色体的制备的影响进行了研究，结果未观察到pha对增加扇贝染色体分裂相有显著的影响。
Together the results show that chemical modification of the lectin can affect heamagglutination and mitogenic activity separately . this indicates that the polygonatum cyrtonema hua . lectin ii has at least two active centres which play different roles in determining its activity 研究黄精凝集素n抗艾滋病毒fa琴1和hi从2对cem ( t细胞淋巴瘤细胞系)和th . 4 (小鼠神经细胞系)的感染中的作用发现。
Among these were sequences which encode putative signalling molecules or transcription factors , sequences which detoxify reactive oxygen species , two c - type lectin sequences , ests which encode membrane - associated proteins and seven distinct late embryogenic abundant ( lea ) sequences 这些序列中包括编码信号分子或转录因子、解毒活性氧组分、两个c型凝集素、编码膜相关蛋白的序列以及7个不同的晚期胚胎富有的序列。
Calli were induced on murashige - skoog ( ms ) medium containing 0 . 5mg / l to 2 . 0 mg / 1 2 , 4 - dichlorophenoxyacetic acid ( 2 , 4 - d ) and 0 . 5 rag / 1 to 1 . 0 mg / 1 6 - benzylaminopurine ( 6ba ) . a high frequency of callus induction was obtained on ms medium supplemented with 2 . 0 mg / 1 2 , 4 - d and 1 离体再生黄精根状茎中黄精凝集素的pcr扩增及序列分析表明：其与野生型凝集素比较在核苷酸序列上有2处突变，蛋白质水平上有两处氨基酸变异，分别是55位p突变为q和119位q突变p 。
Crotalaria mucronata lectin ( cml ) was purified from seeds of crotalaria mucronata by extraction , fraction with ( nhi ) 2864 , hog gastric mucin - sepharose 4b affinity chromatography and followed by gel filtration of sephacryl s - 200 hr . cml agglutinated type a human red blood cells specially . the purified cml gave one band pel e ' ectronhoresis and on sds nolvacrvlamide gel electrophoresis 野花生豆( crotalariamucronata )经磨粉、浸取、硫酸铵分级沉淀、猪胃粘蛋白- sepharose4b亲和层析、 sephacryls - 200hr分子筛层析可得到一表观分子量为103kd且对a型血红细胞专一凝集的野花生豆凝集素( crotalariamucronatalectin ， cml ) 。
Abstract : numerous mushrooms are toxic to insects . to identify the chemicals involved in insecticidal activity , the toxicity of 14 species has b een studied for water solubility , thermolability , and dialysis . the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects . among proteins , lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease 文摘:许多蘑菇都对昆虫表现出毒性.为了证实与杀虫毒性有关的化合物,对14种蘑菇的毒性在水溶性、热敏性和可透析性等方面进行了研究.研究数据表明,蛋白质对大多数蘑菇子实体的杀虫活性起着重要作用,也许是一种可以用于植物抵抗害虫的基因源.在数种蛋白质中,凝集素和溶血素因不受蛋白酶的影响而成为良好的杀虫剂候选材料
A man / glc - specific dolichos puepureus lectin ( dpl ) was purified from seeds of dolichos puepureus l . var . by a procedure including precipitated by ammonium sulphate , deae - 52 iron - exchange and sephacryl s - 300 hr gel filtration . the purified dpl showed a single band on page and ief gel , two bands on sds - page under reducing and non - reducing congditions 黑皮扁豆( dolichospurpureusl . var . )经粉碎、浸取、硫酸铵分段盐析、 deae - 52离子交换和sephacryls - 300hr分子筛层析可得到一表观分子量为155kd的甘露糖葡萄糖专一的黑皮扁豆凝集素( dolichospurpureuslectin ， dpl ) ，无血型专一性。
The hemagglutinating activity of amphioxus skin mucus was decreased after heating at 60 for 30 min , and was almost lost upon heating at 100 for 30 min . the hemagglutinating activity of the skin mucus remained stable in the ph range from 6 to 10 , but it was inactivated at ph above 10 or below 6 . the hemagglutinating activity in the skin mucus is inhibited by d - galactose , which is same with the hemagglut inating activity of the humoral fluids of amphioxus 在60保温30分钟后，凝集素的凝集活性下降，在100保温30分钟后，凝集素凝集活性几乎完全消失；在ph6 - 10的范围内，凝集素的凝集活性稳定， ph大于10小于6的条件下，凝集素的凝集活性消失；文昌鱼体表粘液中的凝集素受d -半乳糖苷的抑制。
The xerocomus spadiceus lectin , xsl , was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation , anion exchange chromatography on deae - cellulose , affinity chromatography on affi - gel blue gel , cation exchange chromatography on cm - cellulose , and gel filtration by fast protein liquid chromatography on superdex 75 从砖红绒盖牛肝菌( xerocomusspadiceus )子实体粗提物中，经过deae -纤维素阴离子交换层析、 affi - gelbluegel亲和层析、 cm -纤维素阳离子交换层析和superdex75fplc凝胶过滤，纯化了砖红绒盖牛肝菌凝集素。
In order to investigate the role of mannose receptor ( mr ) of human sperm , the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography . the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope , respectively . the mice were immunized with pmr and the antiserum was raised . after capacitation and induction of the acrosome reaction , the human spermatozoa and oocytes were incubated with the antiserum . then the sperm penetration assay was undertaken 为了进一步探讨人精于mr在精卵融合中的作用，本文采用改良后的甘露糖-琼脂糖凝胶亲和层析法分离纯化人精子mr ，并将提纯的人精子甘露糖受体( purifiedmannosereceptor , pmr )作用于去透明带的金黄地鼠卵母细胞，运用透射电子显微镜技术和罗丹明偶联的兵豆凝集素( tritc - lca )免疫荧光标记技术观察pmr对卵子的影响。
