The main contents are as followings : ( 1 ) the rules of choosing characteristic lines of fe and ni elements in the cu - pb alloy are investigated ; ( 2 ) the characteristic line of the impurity element and that of the reference element makes up a line pair . the intensity ratio of the line pair measured as an y - axis and the corresponding concentration ratio as x - axis , calibration curves for composition analysis is fitted at the 4 kinds of buffer gases Q ）采用内标法，由样品中杂质元素的分析线和内标元素参考线组成分析线对，对五种不同的铅黄铜样品，实验通过测定了杂质元素分析线队的强度比，以分析线对的强度比为纵坐标、分析线对所对应元素的含量比为横坐标，拟合出了一系列在四种缓冲气体中的定标曲线。
According to the gene sequence and secondary structure of hcv ns5b , we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et . al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion , the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr 然后根据dsrna设计原则，结合nssb基因的序列特征，借助生物信息学软件设计了针对nssb基因的sirnas ，并交由公司化学合成；电穿孔法转染上述稳定转染的细胞克隆，同时分别以非特异的sirnas转染组和空白转染组为对照， dapi染色后通过荧光显微镜和内标化rtpcr检测，初步证实了化学合成的sirnas可以特异阻断nssb基因的表达。
K01458 , 1284bp ) , we designed the primers and regard the pcr productions as the internal standard . then by using rt - pcr analysis , high levels of expression of emu ghrelin mrna were detectable in proventriculus were detected , low levels of expression of duck ghrelin mrna were also detectable in lung muscle ileum duodenum corpus striatum cerebellum brain stem and gizzard K01458 ， 1284bp )设计引物，以其pcr产物作为内标，分别以鸸鹋各个组织反转录产物为模板，通过多重pcr的检测ghrelin的mrna在鸸鹋的各个组织中表达情况，结果发现ghrelin的mrna在鸸鹋ghrelin的mrna除在腺胃特异表达外，在肺、肌肉、回肠、十二指肠、纹状体、小脑、脑干、肌胃也有少量表达。
