By immunizing rabbits with aa 450 - 565 or aa 385 - 730 fragment , polyclonal sera were obtained that were able to recognize e2 proteins expressed in both e . coli and mammalian cells , and cross - react between certain hcv subtypes 利用aa450 - 565和aa385 - 730两片段免疫家兔，获得了可识别大肠杆菌和哺乳动物细胞表达之e2蛋白的多抗血清，并且在某些亚型之间具有一定程度的交叉反应性。
Using agarose gel double diffusion method , the antiserum of pea ferritin can cross - react with the crude extraction of soybean seed ferritin . the result indicated that high specific antiserum had been acquired and could be used ~ in the detection of the expressi 5 、植物铁蛋白的免疫学检测琼脂糖免疫双相扩散法显示，制备的豌豆铁蛋白抗血清可与大豆铁蛋白粗提物发生免疫交叉反应。
But the extensive cross - reactivity and the lacks of the ideal antisera make it difficult in technique and the false result . furthermore , the lack of the standard antisera also cause the " blank " result in a few newly discovered alleles 但血清学方法不仅存在着广泛的交叉反应和难以找到理想的抗血清而出现技术上的困难和分型判断误差，还会有许多新发现的等位基因却缺乏相应的标准抗血清，导致个别基因型无法检出。
Although cases of cross - reactivity ( actually developing an allergy from a sulfonylurea ) are rare , if you have a severe allergic reaction ( hives , breathing problems , or severe rash ) , it would be better for you to use a different medication than a sulfonylurea , if you can use one that is as effective 尽管药物交叉反应的现象很罕见（实际上这个过敏可能就是由磺酰脲类药物引起的） ，如果你出现严重的过敏反应（如麻疹，呼吸困难，或者严重的疹子） ，那么你最好换一种降糖药，不要选择磺酰脲类降糖药，当然换的药物要可以有效降低你的血糖才行。
Serious cross reation existed between v . albo - atrum and mv2 , mv3 , mv4 . the other pathogen isolates v31 and v32 also had cross reactions , but the reaction was not serious . because limited number of pathogen isolates were selected , it could not prove that the selected immunogen was widely presentative , more pathogens isolates should be tested to verify the acquired hybridomas cells 5株单抗杂交瘤细胞中没有一株具有种或属的特异性，其中mv2在棉花黄萎病菌若干菌系间的检测表明其能够区分不同的致病类型； mv1和mv4组合检测的结果基本上能将棉花大丽轮枝菌鉴定到种；黑白轮枝菌与mv2 ， mv3 ， mv4的交叉反应比较强烈，其他菌株v3 ， v32有个别的交叉反应，但不强烈
Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus . for higher plants , however , though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes , only limited evidence is available for the presence and properties of the ip3r - like protein so far . here , using the immunological methods with an antibody raised against a mammalian inositol 1 , 4 , 5 - triphophate receptor ( type 1 ) , we found that , 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively 本实验用sds - page电泳和免疫印迹的方法，用哺乳动物大鼠三磷酸肌醇受体的多肽做抗体对类三磷酸肌醇受体蛋白鉴定，结果表明：抗体与水稻和拟南芥微粒体蛋白分子量大约为200kd的蛋白交叉反应，同时还发现在水稻微粒体蛋白62kd和拟南芥微粒体蛋白45kd处有交叉反应的蛋白条带存在，表明在植物中有类三磷酸肌醇受体蛋白的存在；用免疫胶体金方法，发现类三磷酸肌醇受体蛋白主要分布于液泡膜和细胞质膜上。
The high specificity of dot - ppa - elisa was proved by the specific blocking test , and also by the cross - reaction test in which the diaphragm did n ' t react with the antibodies against pasteurellosis , streptococcosis , colibacillosis , chlamydiosis , hcv , ppv , brucellosis , prv and foot - mouth disease . the diaphragm has good sensitivity and could detect some salmonella - positive test serum which has been diluted to 1 : 2048 . stored at 4 for at least 6 months or at 10 - 25 " c for 4 months , the sensitivity and specif icity of the diaphragm did n ' t change , so it has good stability 本研究制备的诊断膜片特异性强：不与猪衣原体病、猪口蹄疫病、猪大肠杆菌病、猪布氏杆菌病、猪瘟、猪伪狂犬病、猪细小病毒病、猪巴氏杆菌病、猪链球菌病的阳性血清发生交叉反应；诊断膜片具有良好的敏感性，能够检测到1 ： 2048稀释的动物试验阳性血清；膜片的保存期长，在10 25可保存4个月、 4条件下至少可保存6个月其灵敏度不变。
No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page , except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ) , which is a selective marker of the wild - type virus . elisa test results suggested the expression of egf in cells , but not in culture supernant . the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract 将重组病毒rbmbacph - egf以10moi感染bmn细胞， 72小时后收集培养细胞和上清；培养上清和经超声波处理的细胞样品elisa检测发现胞内样品中存在能与egf抗体免疫反应的产物，粗略估计表达量约6 7 g 2 10 ~ 6个细胞(相当于egf标准品) ；重组病毒rbmbacph - egf穿刺接种5龄家蚕幼虫，每隔24h收集蚕血淋巴，经elisa检测发现第4天表达量最高，根据egf标准曲线计算蚕血淋巴的表达量约32 g ml ； elisa定性实验还发现正常蚕血也存在与egf抗体间交叉反应的物质。
With anti - t - jgy establishing a radioimmunoassay , the quality of anti - t4 igy was appraised : litres was 1 : 25 00 , permitting rang of litres in the ria was from 1 : 2500 to 1 : 5000 ; the degree of cross reactivities with ta was 1 . 28 % ; affinity constants was 2 . 13 x 109l / mol 本文应用蛋黄正丁醇去脂获得的抗t4 igy建立了放免检测方法，对抗t4igy的质量进行鉴定：抗体滴度为1 ： 2500 ，工作浓度在1 ： 2500至1 ： 5000范围内；与t3的交叉反应为1 28 ；亲和常数为2 13x109l m 。
The high specificity of the dot - ppa - elisa was confirmed by specific blocking test and also by cross - reaction test . the diaphragm did not react with the antibodies against salmonellosis , streptococcosis , colibacillosis , chlamydiosis , hcv , ppv , prv , brucellosis . erysipelas , suis and chlamydiosis in cross - reaction test . the diaphragm has good sensitivity and could detect some pasteurella - positive test serum which has been diluted to2 - " 试验证明所建立的dot - ppa - elisa具有较好的特异性，与猪瘟、仔猪副伤寒、猪丹毒、猪细小病毒病、猪伪狂犬、猪布氏杆菌病、猪衣原体病阳性血清无交叉反应。
