A series of validation experiments and genetic studies should be performed for the y - str multiplex system according to the suggestion of the technical work group dna analysis methods ( twgdam ) . method we selected four y - str loci , dys434 , dys438 , dys439 , a10 ( y - gata - a10 ) and designed two set of tailed primers to improve the efficiency of the multiplex pcr 方法选定四个y染色体str基因座，应用加尾序列引物设计策略设计的引物，构建四个基因座的y - str复合扩增体系，建立银染检测和荧光检测方法，依据dna分析技术工作组( twgdam )指南进行法医学可行性研究和群体遗传研究。
Especially for the study of chinese y chromosome diversity , disease distribution , the origin of the nationality han , and flow disciplines , the distribution data and areas of the top 300 common chinese names would provide new clues and information which have the highest valuation in research and practical use 尤其是中国人前300个常见姓氏的数据和分布地区，对于研究中国人y染色体多样性疾病的分布汉民族源和流以及其他学科领域，都有可能提供新的线索和参考，具有极高的研究和实际使用的价值。
Y chromosome is transmitted in the form of hap - loid , leading to extreme disequilibrium of y chromosome genetic markers distribution in different population . the prerequisite of str application in forensic medicine is establishment of a database of population y - str loci haplotype distribution . therefore we need to form haplotypes by using the known highly polymorphic str loci and detect more local population 由于y染色体呈单倍体遗传，导致y染色体遗传标记在不同人群中的分布极不平衡，群体差异比常染色体str位点更加显著，在法医学应用的前提条件是：建立含有多个y - str位点的单倍型的群体分布数据库。
