A combined metabolic mutated strain ( ys - c ) was get by traditional filter method from corynebacterium glutamicum ( ys ) . this mutant is threonine auxotrop , aec and ahv resistant , and it can grow in the medium which contants sca as the only source of carbon , and the mutant can endure hight leveal of leu . the mutanted strain can concentrate lysine . the concentration of lysine leveal was 1 . 20g / l 运用传统育种手段筛选到谷氨酸棒杆ys的多重代谢突变组合菌株ys - c 。该菌株为苏氨酸营养缺陷型，具有对赖氨酸的结构类似物aec和ahv的抗性，并且能以琥珀酸为唯一碳源生长，能够耐受高浓度亮氨酸。
