The second part of this paper takes the horizontal theory as the tool to introduce and comment several kinds of representative humanity theory . they include the theory of original goodness of human nature represented by confucius and mencius , the theory of original evil of human nature declared by xunkuang and hanfei , the theory of non - original human nature claimed by gaobuhai wangling and sushi , the theory of original goodness and evil of human nature represented by shishuo yanxiong and simaguang , the theory of three kinds of original human nature , the theory of original dualism human nature claimed by zhangzai ercheng and zhuxi , and egoism altruism and the theory of egosim and altrusim prevailing in the west 文章第二部分是以平面理论为工具对几种有代表性的人性理论进行介绍和评述的，包括以孔子、孟子为代表的性善论：以荀况、韩非为代表的性恶论；以告不害、王令、苏轼为代表的性本无论；以世硕、扬雄、司马光为代表的性善恶混说；以董仲舒、王充、荀悦为代表的性三品论；以张载、二程、朱熹为代表的性二元论以及流行于西方的利己主义、利他主义和己他两利主义。
In this experiment , radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process . methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0 . 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al . thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum , and added to 50ml cell culture flasks 方法：胰岛的分离参照王玲等的方法，每次实验取新生3 - 5天sd大鼠，无菌条件下剖腹取出胰腺，剪切为0 . 5mm ~ 3的组织块， v型胶原酶消化30min后，离心洗涤，悬浮于完全培养基，接种入50ml培养瓶，于5 co _ 2 、 95空气条件下培养20h ，转板纯化，接种于96孔培养板培养24h ，按实验要求进行实验。
