Clone human wnk4 full length cdna into pgem - t vector human kidney total una was extracted and used as template to amplify wnk4 full length cdna with the forward primer ( 7 - 27 ) and reverse primer ( 3808 - 3833 ) with the long template expanded pcr system kit 5 .原位杂交以小鼠肾脏总翩a为模板，扩增wnki基因外显子1 ， 24一28 ，以a片段和wnk4基因外显子13一16片段，纯化后连接在pgem一t载体上。
The tension is both alluring and breathtaking . dubbed " the world ambassador for argentina s national social dance " , miguel angel zotto dares you to join his critically acclaimed dance company tango por dos for their compelling new show tango una leyenda 佐图艺高胆大，以首席舞者和艺术总监的身份带领舞团tango por dos上演探戈传奇，如数家珍地展现探戈的百年旖旎风采，让观众看尽探戈的千娇百媚，也见证阿根廷社会风貌的沧桑变化。
The tension is both alluring and breathtaking . dubbed " the world ambassador for argentina s national social dance " , miguel angel zotto dares you to join his critically acclaimed dance company tango por dos for their compelling new show tango una leyenda ?佐图艺高胆大，以首席舞者和艺术总监的身份带领舞团tangopordos上演《探戈传奇》 ，如数家珍地展现探戈的百年旖旎风采，让观众看尽探戈的千娇百媚，也见证阿根廷社会风貌的沧桑变化。
The parents of 8 - year - old martha and 4 - year - old linda have gone elsewhere to earn living for the family and their aunt una , unmarried and about thirty years old , is asked to look after them . under aunt una s strict supervision , the two sisters begin to learn about the secrets of compromise 玛花和莲达两姊妹的父母要到外地工作，于是请了未婚的云娜阿姨来照顾她们，淘气反斗的玛花与古板严厉的云娜姨简直水火不容，两人究竟可以如何相处？
The parents of 8 - year - old martha and 4 - year - old linda have gone elsewhere to earn living for the family and their aunt una , unmarried and about thirty years old , is asked to look after them . under aunt una s strict supervision , the two sisters begin to learn about the secrets of compromise 玛花和莲达两姊妹的父母要到外地工作，于是请了未婚的云娜阿姨来照顾她们，淘气反斗的玛花与古板严厉的云娜姨简直水火不容，两人究竟可以如何相处？
